应激系统对缺血预适应心肌细胞凋亡的影响  

Stress response modulate the apoptosis of ischemic preconditioning on rabbits hearts

作　　者：许华山[1] 陈胜喜[1] 罗万俊[1] 蒋海河[1] 袁明道[1] 杨扬[1] 朱国勇[1] 

机构地区：[1]中南大学湘雅医院心胸外科,湖南长沙410008

出　　处：《中国医学工程》2006年第6期571-575,共5页China Medical Engineering

摘　　要：目的探讨应激系统对缺血预适应中心肌细胞凋亡的影响。方法以家兔在体心经典缺血预适应(IPC)实验为基础,增设结扎肾上腺组(Lig组),米非司酮组(皮质醇受体水平阻滞剂)(MF组),甲基强的松龙组(MP组),以TUNEL法,DNAladder及流式细胞仪(FASCan)检测凋亡的存在及凋亡率,同时检测血清皮质醇动态变化,考察应激启动的皮质醇分泌对缺血预适应心肌细胞凋亡的影响。结果TUNEl染色显示MP组和IPC组比较凋亡率差异没有显著性意义(5.05±2.25%vs3.46±0.82%,P>0.05),MF组和Lig组与IPC组比较差异有显著性意义(7.73±3.08%,16.62±12.35%vs3.46±0.82%,P<0.05);DNAladder半定量显示MP组和IPC组电泳条带明显减少;流式细胞仪检测显示Lig组,MF组心肌细胞凋亡较IPC组差异有显著性意义(3.82±1.38%,3.22±0.67%vs1.72±0.29%,P<0.05),MP组与IPC组比较差异没有显著性意义(2.04±0.74%vs1.72±0.29%)。结论从细胞凋亡角度观察IPC的心肌保护作用,结扎肾上腺,受体水平阻滞皮质醇作用可明显削弱其保护作用,MP可一定程度模拟其保护作用,说明应激系统的部分组分通过影响IPC的心肌细胞凋亡起到其保护作用。[Objective] To study the act of stress system on cardiomyocyte apoptosis of ischemic preconditioning （IPC）. [Methods] Based on classic IPC research operation using rabbit heart, 5 groups were set with 3 adding groups. （Bilateral ligated adrenal group （Lig）, mifiprestone group （MF）, and a methylprednisolone group （MP）. using TUNEL dyeing method, DNA ladder and FACScan to study the rate of apoptosis of the ischemic cardiomyocyte. [Result] By TUNEL dyeing, the difference of apoptosis ratios between MP and IPC group was insignificant （5.05±2.25% vs 3.46±0.82%, P 〉0.05）, comparing with IPC group, the difference of MF and Lig group was significant （7.73±3.08%, 16.62±12.35% vs 3.46±0.82%, P〈0.05）. by FACScan, the difference of apoptosis ratio between IPC and MP group was also insignificant （2.04±0.74% vs 1.72±0.29%, P 〉0.05）, comparing with IPC group, the difference of Lig group and MF group was significant （3.82±1.38%, 3.22±0.67% vs 1.72±0.29%, P 〈0.05）, and the DNA ladder also showed a decrease in IPC and MP group comparing with IR, Lig, and MF group. [Conclusion] stress system plays an important role during the IPC process on cardiomyocyte apoptosis.

关 键 词：缺血预适应 细胞凋亡 皮质醇 米非司酮 心肌保护 

分 类 号：R-332[医药卫生] R542.2