法夫酵母诱变选育高产虾青素菌株的研究  

Study on astaxthin-overproducing mutants of Phaffia rhodozyma mutagenesis

作　　者：谢虹[1] 周元元[1] 夏小伟[1] 梁建生[1] 

机构地区：[1]扬州大学生物科学与技术学院,江苏扬州225009

出　　处：《扬州大学学报（农业与生命科学版）》2006年第4期80-84,共5页Journal of Yangzhou University：Agricultural and Life Science Edition

基　　金：江苏省高校自然科学基金(05KJB180157)

摘　　要：以法夫酵母(Phaffia rhodozyma)为试验材料,对其先进行紫外线和氯化锂(UV-LiCl)复合诱变处理,获得了一株产虾青素为635．82μg·g^(-1)的菌株UL-40,其中紫外线适宜诱变条件为30 W、20 cm处照射菌株3 min,LiCl筛选剂量为3 g·L^(-1)。对UL-40菌株用N-甲基-N′-硝基-N-亚硝基胍(NTG)和2-脱氧-D-葡萄糖再进行复合诱变处理,获得了1株遗传性状稳定、25℃条件下的产虾青素为894．54μg·g^(-1)菌株ND-25,其中NTG在25℃条件下最适诱变剂量为150μg·mL^(-1),2-脱氧-D-葡萄糖最适筛选剂量为1 g·L^(-1)。Mutagenesis of Phaffia rhodozyma was attempted with UV and NTG. Adaptive dosage of UV was 30 W, 20 cm, 3 min, and adaptive dosage of NTG was 150 μg · mL^-1 under 25 ℃ and 30 min. In the selection of astaxthin-overproducing mutants of P. rhodozyma, 2 yeast growth inhibitors, LiCl and 2-deoxy-D-glucose were tested and their adaptive dosage were 3 g · L^-1 and 1 g · L^-1. Firstly, strain UL-40 was obtained with UV-LiCI compound mutagenesis, which contained astaxanthin 635.82 μg · g^-1 CDW （cell dry weight）. Strain ND-25 was screened with NTG-2-deoxy-D-glucose compound mutagenesis. The results showed that strain ND-25 had better stability and 894. 54 μg · g^-1 CDW capacity of astaxanthin production under 25 ℃ incubation.

关 键 词：法夫酵母 诱变 筛选 虾青素 

分 类 号：Q939.9[生物学—微生物学]