Survivin ASODN对胃癌细胞株SGC-7901增殖的影响  

Effects of survivin ASODN on proliferation in gastric cancer cell line SGC-7901

作　　者：殷晓春[1] 庞春艳[1] 陈卫[1] 

机构地区：[1]兰州大学基础医学院医学生物化学与分子生物学研究所,甘肃兰州730000

出　　处：《中华肿瘤防治杂志》2006年第24期1856-1859,共4页Chinese Journal of Cancer Prevention and Treatment

摘　　要：目的:观察Survivin基因反义寡脱氧核苷酸(ASODN)对胃癌细胞增殖的影响。方法:靶向Survivin ASODN经脂质体介导转染胃癌细胞系SGC-7901,倒置显微镜和电镜观察转染后细胞形态学变化;噻唑蓝(MTT)法检测细胞的增殖情况;半定量RT-PCR检测Survivin基因mRNA的表达;免疫组化检测Survivin蛋白的变化情况;FCM检测细胞周期的变化。结果:转染ASODN后,细胞出现了形态学变化;MTT实验检测发现细胞株SGC-7901增殖受到显著抑制,抑制率达(69．2±0．76)％;RT-PCR检测发现Survivin基因mRNA水平表达显著下降,免疫组化试验发现蛋白表达也出现类似的结果;流式细胞仪检测发现G1期细胞比例增高,由对照组的66％和62．5％增至90．7％。结论:Survivin ASODN可以显著抑制Survivin的表达,抑制胃癌细胞增殖。OBJECTIVE：To investigate the effects of survivin antisense oligodeoxynucleo-tide （ASODN） on proliferation in gastric cancer cell line SGC-7901. METHODS： The gastric cancer cell line SGC-7901 was transfected with survivin ASODN by using Lipofectin box. Morphological changes of the tumor cells were examined by inverted microscope and electron microscope. The proliferation of the cells was determined by MTT method . The change of survivin mRNA of the SGC-7901 cells was detected by RT-PCR assay, and the expression of survivin protein of the cells was measured by the immunhistochemistry assay. RESULTS： The typical morphological changes of apoptosis induced by survivin ASODN in SGC-7901 cells were observed. The proliferation of tumor cells was significantly inhibited, and the growth rate in transfected cells was down to （69.2±0.76） of the normal cells . Survivin ASODN induced downregulation of the survivin mRNA of SGC-7901 cells by RT-PCR measure, and the survivin protein of SGC-7901 cells had the same change by the immunhisto-chemistry assay. The percentage of G1 cellls increased by FCM. CONCLUSION： Antisense ODN targeting survivin gene could significantly inhibit the expression of survivin mRNA and protein, and inhibit the proliferation of SGC-7901 cells.

关 键 词：胃肿瘤 基因 Survivin 细胞系 肿瘤 寡核苷酸类 反义 基因 肿瘤抑制 

分 类 号：R735.2[医药卫生—肿瘤]