Survivin在白血病细胞中的表达及GM-CSF对其影响的研究  

Expression of Survivin in Leukemia Cells and Influence on It by GM-CSF

作　　者：吴耀辉[1] 邹萍[1] 刘芳[1] 沈汉斌[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院血液病研究所,武汉430022

出　　处：《中国实验血液学杂志》2007年第1期6-9,共4页Journal of Experimental Hematology

基　　金：国家自然科学基金资助项目;编号30370595

摘　　要：本研究旨在探讨凋亡存活蛋白survivin在白血病的原代细胞和白血病细胞系中的表达情况,并观察粒-巨噬细胞细胞集落刺激因子(GM-CSF)对其的影响。用RT-PCR方法检测37例白血病患者、10例正常成人骨髓和3种白血病细胞系(K562、HL-60、U937)中survivin的表达。以HL-60细胞为对象,加入合适浓度的GM-CSF,用RT-PCR和Western blot分别检测加药前后surivin mRNA和蛋白水平的变化。结果显示:37例白血病患者中有25例表达survivin,阳性率为67.6%,其中急性淋巴细胞白血病(ALL)细胞中survivin mRNA表达阳性率为73.3%,高于急性髓系白血病(AML),但均高于正常对照组(20.0%)(P<0.05)。3种细胞系K562、HL-60和U937全部表达survivin。合适浓度的GM-CSF作用2天后,HL-60细胞中survivin的表达明显升高,其中mRNA水平升高了26%,蛋白水平升高了49%。结论:suvivin在白血病的原代细胞和细胞系中均有较高表达,而GM-CSF能显著提高HL-60细胞中survivin的水平。This study was aimed to explore the expression of survivin in leukemia cells and to investigate the effect of GM-CSF on survivin expression. The survivin expressions in 37 previously untreated leukemia patients and 10 normal persons as well as in three kinds of leukemia cell Lines （ K562, HL-60, U937） were analyzed by RT-PCR, The HL-60 ceils were treated by the proper concentration of GM-CSF, and the changes of survivin mRNA and protein expression levels were detected by using RT-PCR and Western blot respectively, The results indicated that the positive rate of survivin gene in 37 leukemia patients was 67.6% （25/37） and significantly higher than that in normal control （20, 0% ）. The expression level was higher in ALL ceils than that in AML cells （73.3% vs 63.6% ）. Moreover, three kinds of leukemia cell Lines all expressed survivin. After treating with the proper concentration of GM-CSF for 2 clays, the expression of survivin obviously increased and the expression level of survivin mRNA was up-regulated by 26%, the expression level of survivin protein was up-regulated by 49%. It is concluded that the survivin gene extensively express in leukemia and its cell Lines, and its expression can be obviously increased by GM-CSF.

关 键 词：白血病细胞 SURVIVIN 粒-巨噬细胞细胞集落刺激因子 

分 类 号：R733.7[医药卫生—肿瘤]