多参数流式细胞术观察CD36在白血病细胞上的表达及其意义  

CD36 Expression in Leukemia Cells Checked with Multi-parameter Flow Cytometry and Its Significance

作　　者：唐华容[1] 王法春[1] 江云伟[1] 费霞[1] 蒋茜[2] 许文林[1] 林江[1] 

机构地区：[1]江苏大学附属人民医院中心实验室,镇江212002 [2]江苏大学附属江滨医院检验科,镇江212002

出　　处：《中国实验血液学杂志》2007年第1期29-34,共6页Journal of Experimental Hematology

摘　　要：本研究旨在探讨CD36在白血病细胞的表达及其在白血病诊断和鉴别诊断中的意义。采用CD45/SSC参数设门多色流式细胞术分析133例白血病细胞CD36及其它白血病相关抗原的表达情况。结果表明:133例白血病中有29例患者(21.8%)的白血病细胞表达CD36,62例急性髓系白血病(AML)中26例患者(41.9%)白血病细胞表达CD36,54例淋系白血病未见CD36阳性的病例。M4、M5和M6中CD36的阳性率(8/10、12/12、3/3)明显高于M1(0/9)、M2(3/12)、M3(0/16),具有统计学差异(P值均<0.001);CD36在M5b组的阳性细胞百分数(中位数89·6%)明显高于M5a组(中位数32.7%)(P=0.001)。在急性髓系白血病中,CD36的表达与CD117呈负相关(r=-0.751,P=0.005),与HLA-DR、CD34不相关;在M4和M5b组中CD36与CD14的表达呈正相关(r=0·870,P=0.011);在单核细胞相关性白血病(MLIL)中CD36阳性率为92.6%(25/27),CD14的阳性率为48·1%(13/27),前者明显高于后者(P=0.001),在M5a中CD14未表达,在M5b中CD14表达为100%(5/5);CD117在M5a的阳性率明显高于M5b(P=0.010);CD34在M5的阳性率较低(33.3%,4/12)。结论:结合CD36与其它淋系及髓系抗原有助于淋巴系白血病、髓系白血病及其单核细胞相关性白血病的诊断与鉴别,其在单核细胞相关性白血病阳性率为92.6%(25/27),高于CD14(41.8%,13/27)。The aim of study was to investigate the expression of CD36 in leukemia cells and to explore its significance in diagnosis and differntial diagnosis for leukemia in patients. Blood samples from 133 cases of leukemias were analyzed by CD45/SSC double parameters and multi-color flow cytometry in order to determine the CD36 and other leukocyte differentiation antigens. The results show that the CD36 positive rate was 21.8% （29/133） in 133 cases of leukemia, 41.9% （26/62） in 62 cases of AML（ acute myeloid leukemia）, and none of the 54 cases of lymphocytic leukemia was positive for this antigen. The positive rate of CD36 in M4（8/10） , M5（12/12） and M6（3/3） was significantly higher than that in M1 （0/9）, M2 （3/12）, M3 （0/16） （ all P 〈 0. 001 ）. The percent of positive cells of CD36 in M5a was significantly higher than in Msb （ P = 0. 001 ）. A significantly negative regression was found between CD36 and CD117 in AML（ r = -0.751, P = 0. 005 ）. And a significantly positive regression was found between CD36 and CD14 in M4 and M5b （r = 0. 870, P = 0.011 ）. In monocyte lineage involved leukemia （MLIL）, the positive rate of CD36 （ 92.6%, 25/27 ） was significantly higher than that of CD14 （ 48.1%, 13/27） （ P = 0.001 ）. None of the 7 cases with Ms, was positive for CD14, but 4 of 5 cases of Msb were positive. The positive rate of CD117 in Ms, was significantly higher than that of in Msb（P =0.01）. The positive rate of CD34 in M5 was low （33.3% ,4/12）. It is concluded that the combination of CD36 with lymphoid and myeloid antigens is helpful to the diagnosis and differential diagnosis of lymphoid, myeloid and MLIL. The positive rate of CD36 is higher than that of CDI4 in MLIL.

关 键 词：白血病 CD36 免疫表型 多参数流式细胞术 

分 类 号：R733.7[医药卫生—肿瘤]