急性白血病LRP15基因启动子区甲基化及其表达分析  

LRP15 Gene Promoter Region Methylation and Its Expression in Acute Leukemia

作　　者：赵瑜[1] 窦丽萍[1] 王全顺[1] 卢学春[1] 楼方定[1] 于力[1] 

机构地区：[1]解放军总医院血液科,北京100853

出　　处：《中国实验血液学杂志》2007年第1期39-41,共3页Journal of Experimental Hematology

基　　金：国家自然科学基金(编号39970318);973国家重点基础研究专项经费资助项目(编号2005CB522400)

摘　　要：为了探讨LRP15基因在急性白血病(AL)患者中的表达情况及其与启动子区CpG岛甲基化的关系,采用甲基化特异PCR(MSP)方法检测AL患者LRP15基因启动子区甲基化状态,并用RT-PCR方法检测该基因在这些患者中的表达情况。结果发现,LRP15基因在缓解与非缓解状态白血病患者中的表达分别为47.6%和16.7%,其启动子区甲基化比例分别为38.1%和72.2%。LRP15基因的表达与其启动子区甲基化之间无相关(P=0.0087)。结论:AL患者LRP15基因启动子区的甲基化不完全是导致LRP15基因表达沉默的原因。To investigate the relationship between LRP15 gene promoter region methylation and its gene expression in acute leukemia patients, the status of LRP15 gene promoter region methylation was detected by MS-PCR and the gene expression was detected by RT-PCR in bone marrow samples from leukemia patients. The results indicated that the LRP15 gene expression was 47, 6% in complete remission （CR） patients and 16.7% in non-CR patients respectively, while LRP15 gene promoter region methylation was 38.1% in CR group and 72, 2% in non-CR group respectively. No relationship was found between LRP15 gene promoter region methylation and its expression （P = 0. 0087）. It is conclu- ded that the methylation in LRP15 gene promoter region may not be the only reason for LRP15 gene silence,

关 键 词：LRP15基因 急性白血病 启动子区甲基化 

分 类 号：R733.71[医药卫生—肿瘤]