存活蛋白和P63蛋白在B细胞非霍奇金淋巴瘤组织中的表达及其对细胞凋亡和增殖的作用  

Expression of Survivin and P63 Protein in B Cell Non-Hodgkin's Lymphoma and Their Effects on Cell Apoptosis and Proliferation

作　　者：左学兰[1] 周颖[1] 周新[2] 刘小红[1] 张克俭[1] 杨华强[1] 孟娟[1] 

机构地区：[1]武汉大学中南医院血液科 [2]武汉大学中南医院基因检测中心,武汉430071

出　　处：《中国实验血液学杂志》2007年第1期99-102,共4页Journal of Experimental Hematology

基　　金：湖北省自然科学基金资助项目(编号2001ABB159)

摘　　要：本研究探讨存活蛋白(Survivin)和P63蛋白在B细胞非霍奇金淋巴瘤(B-NHL)发生发展中的可能作用及其与细胞凋亡和增殖的相互关系。应用免疫组织化学SP法和TdT介导的dUTP缺口末端标记(TUNEL)技术检测43例B-NHL和10例反应性增殖淋巴组织(RHL)中的survivin和P63蛋白表达以及细胞凋亡和增殖细胞核抗原(PCNA)的水平。结果表明:survivin和P63在43例B-NHL中表达的阳性率分别为69.8%(30/43)和82.7%(36/43),survivin和P63在10例RHL中表达阳性率分别为10%(1/10)和40%(4/10),survivin在侵袭性B-NHL中的阳性率明显高于惰性B-NHL(83.3%vs46.2%,P<0.01),P63蛋白表达差异无显著性意义;survivin阳性表达与凋亡指数(AI)和增殖指数(PI)呈正相关(r=0.429,P<0.01;r=0.348,P<0.01),P63蛋白阳性表达与AI和PI呈正相关(r=0.451,P<0.01;r=0.369,P<0.05),B-NHL的AI和PI呈显著正相关(r=0.598,P<0.001)。结论:survivin可能参与了B-NHL的凋亡和增殖调控机制,P63作为癌基因参与B-NHL发生,二者可能共同影响细胞增殖和凋亡。The study was aimed to explore the possible roles of survivin and P63 protein in the development and progression of B cell non-Hodgkin＇s lymphoma （B-NHL） and their relation with cell apoptosis and proliferation. TdT- mediated dUTP nick end labeling （TUNEL） and immunohistochemistry were used to detect the survivin and P63 protein expression, cell apoptosis and proliferating cell nuclear antigen （PCNA） level in 43 cases of B-NHL and 10 cases of reactive hyperplasia lymphoid （RHL） tissues. The results indicated that the positive rates of survivin and P63 protein expression were 69.8% （30/43） and 82.7% （30/43） respectively. The expression of survivin and P63 protein was 10% （1/10） and 40% （4/10） in RHL tissues of 10 cases. The expression of survivin in aggression B-NHL was higher than that in indolent B-NHL （83.3% vs 46. 2%, P 〈0.01 ）. The expression of P63 proteins in aggressive B-NHL was higher than that in indolent B-NHL （ 86.7 % vs 76. 9 %, P 〉 0.05 ）. Apoptotic index （AI） and proliferation index （PI） correlated positively with expression of survivin （ r = 0. 429, P 〈 0. 01 ; r = 0. 348, P 〈 0. 01 ）, and so do with expression of P63 proteins （ r = 0.451, P 〈 0.01 ; r = 0. 369, P 〈 0.05 ）. In addition, AI and PI were positively related （r = 0. 598, P 〈 0. 001 ）. It is concluded that survivin may participate in the regulation mechanism of B-NHL cell apoptosis and proliferation, P63 as an oncogene enhances proliferation and takes part in the development of B-NHL. There may be a close relationship between survivin and P63 protein in the regulation of lymphocyte proliferative kinetics.

关 键 词：B细胞非霍奇金淋巴瘤 SURVIVIN P63蛋白 细胞凋亡 细胞增殖 

分 类 号：R733.1[医药卫生—肿瘤]