慢性粒细胞白血病患者胸腺输出功能与bcr-abl mRNA水平的关系  

Relationship Between Thymus Output Function in CML Patients and Their bcr-abl mRNA Levels

作　　者：耿素霞[1] 杜欣[1] 翁建宇[1] 陈少华[2] 杨力建[2] 李扬秋[2] 

机构地区：[1]广东省人民医院血液科,广州510080 [2]暨南大学医学院血液病研究所,广州510632

出　　处：《中国实验血液学杂志》2007年第1期138-141,共4页Journal of Experimental Hematology

基　　金：国家自然科学基金(编号30270579);广东省自然科学基金重点项目(编号23001);广东省教育厅"千百十工程"优秀人才培养基金项目(编号Q校02022)

摘　　要：本研究了解慢性粒细胞白血病(chronic myelogenous leukemia,CML)患者体内T细胞受体重排删除环(Tcell receptor rearrangement excision circles,TREC)的含量与bcr-abl mRNA水平的关系,进而明确CML患者的胸腺近期输出功能测定对疾病预后判断的意义。实时定量PCR检测15例CML-CP患者外周血TREC含量及bcr-abl融合基因转录本的水平,并追踪检测6例患者bcr-abl水平的变化。结果发现:在CML初发时患者外周血TREC含量与bcr-abl融合基因水平无明显的相关性;随访2年后,TREC含量高的患者bcr-abl水平下降幅度更大,而在接受造血干细胞移植的2例患者中,移植前TREC含量相对较高者,移植1年后连续3次均检测不到bcr-abl,另1例则检测到低水平的bcr-abl。结论:CML患者胸腺输出功能较高者,可能对机体抵抗残留CML细胞有一定的帮助。The study was purposed to analyze the relationship between the content of T-cell receptor excision DNA circles （TREC） and bcr-ab1 mRNA levels in CML patients and to evaluate the prognostic significance of recent thymic output function detection in patients with chronic myelogenous leukemia （ CML ）, Quantitative detection of TREC and ber-abl fusion gene transcripts in peripheral blood from 15 CML patients were preformed by real-time PCR. The change of bcr-ab1 levels in 6 patients was followed-up for two years. The results showed that there was no significant correlation between TREC and bcr-ab1 mRNA levels in peripheral blood from CML patients for the first attack. Patients who had higher TREC at diagnosis had a larger reduction of bcr-ab1 after 2 years of follow-up. While out of 2 patients who underwent haemopoietic stem cell transplantation（ HSCT）, one patient with higher level of TREC before transplantation was confn＇med to express undetectable level of TREC by three consecutive detections after transplartation, other one patient was identified to express low level of bcr-ab1. It is concluded that high thymic output function in CML patients can be beneficial for killing the residual CML cells.

关 键 词：慢性粒细胞白血病 胸腺输出功能 bet-ab1融合基因 TREC 

分 类 号：R733.72[医药卫生—肿瘤]