低剂量辐射后小鼠血清的蛋白质组学研究  

Serum Proteome in Mice after Low Dose Radiation

作　　者：李薇[1] 张一琼[1] 王冠军[1] 王杰 张学敏[2] 

机构地区：[1]吉林大学第一医院血液肿瘤科,长春130021 [2]吉林大学第一医院国家医学生物分析中心,北京100850

出　　处：《中国实验血液学杂志》2007年第1期191-194,共4页Journal of Experimental Hematology

基　　金：国家自然基金资助项目(编号30370439);教育部高等学校博士学科点专项科研基金资助(编号20020183015)

摘　　要：本研究应用蛋白质组学方法探讨低剂量辐射对造血系统的作用机制,筛选与低剂量辐射作用机制相关的蛋白质,为低剂量辐射的临床应用提供实验依据和理论基础。利用双向凝胶电泳建立低剂量辐射后不同时间点小鼠外周血血清与假照射组蛋白质组二维凝胶电泳图谱,利用MALDI-TOF-MS生物质谱对差异表达的蛋白质进行鉴定分析。结果表明:低剂量照射后,与假照射组相比照射组新出现的蛋白点有4个,表达上调的蛋白点有13个,表达下调的蛋白点有6个,消失的蛋白点有3个。某些蛋白的表达在照射组不同时间点呈现动态的变化规律,经质谱鉴定发现雌激素受体在照射组表达下调,维生素D结合蛋白及载脂蛋白等在照射组表达上调。结论:低剂量辐射使血浆中某些蛋白表达上调或下调,并发现了一些与低剂量辐射作用机制相关的蛋白质。This study was purposed to investigate the mechanism of low dose radiation （LDR） by proteomic technology and to find the key proteins of the hormesis and adaptive response induced LDR, which provided the foundation of experimental and theoretical basis for the clinical application of LDR. Two-dimensional electrophoresis （2- DE） was used to screen protein patterns of normal serum and serum of mice exposed to LDR in different time for qualitative and quantitative differences in protein expression. And the differentially-expressed proteins between the two groups were identified by matrix assisted laser desorption/ionization-time of flight-mass spectrometry （MALDI-TOF- MS）. The result showed that among the differentially-expressed proteins between the group exposed to LDR and the control group （shorn-irradiated group）, it was found that after LDR new 4 proteins appeared, 13 proteins were up- regulated, 6 proteins were down-regulated, 3 proteins disappeared in the group exposed to LDR. In different time the quantity of some proteins was different, the protein expression had some characteristics, the estrogen receptor 2 was down-regulated, the vitamin D-binding protein and apolipoprotien were up-regulated in the group exposed to LDR. It is concluded that LDR up-regulate or down-regulate some proteins, some proteins related with LDR were found. It may provide some new explanations for the effect mechanism of the LDR.

关 键 词：蛋白质组 低剂量辐射 二维凝胶电泳 质谱 

分 类 号：R811.5[医药卫生—放射医学] R311.1[医药卫生—临床医学]