异丙酚对大鼠肾脏缺氧及复氧损伤的保护作用  

Propofol Protecting Kidney from Hypoxia/reoxygenation Injury in Rats

作　　者：孙莹杰[1] 高光洁[1] 张铁铮[1] 宋丹丹[1] 王凤学[1] 刘晓江[1] 

机构地区：[1]沈阳军区总医院麻醉科,沈阳市110016

出　　处：《天津医药》2007年第1期52-53,共2页Tianjin Medical Journal

摘　　要：目的:探讨异丙酚对大鼠肾脏缺氧及复氧损伤的保护作用与机制。方法:将Wistar大鼠24只随机分3组:对照组(A组),缺氧及复氧组(B组)和异丙酚预处理组(C组)。采用酶联免疫吸附法检测大鼠肾组织8-异前列腺素F2α(8-iso-PGF2α)水平,硝酸还原法测定一氧化氮合酶(NOS)活力和NO含量。结果:缺氧及复氧后,B组肾组织中8-iso-PGF2α水平明显升高,而NOS活力和NO含量则明显降低,与A组相比差异有统计学意义(P<0.01);C组与B组相比,8-iso-PGF2α水平明显下降,而NOS活力和NO含量明显上升,差异亦非常显著(P<0.01)。结论:异丙酚能明显降低缺氧及复氧后肾组织的8-iso-PGF2α水平,提高内源性NO生成,提示对大鼠肾脏缺氧及复氧损伤有明显保护作用。Objective： To investigate the protective effect and mechanism of propofol on renal hypoxia/reoxgenation （H/R） injury in rats. Methods： Twenty-four Wistar rats were randomly divided into three groups： the control group （group A）, the hypoxia/reoxygenation （H/R） group （group B） and the H/R＋ propofol group （group C）. The 8-iso-prostaglandin F2α（8-iso-PGF2α） levels in the renal tissue were determined by enzyme-linked immunosorbent assay （ELISA）. The contents of NO and the activity of NOS were detected by azotic acid disoxidation methods. Results： 8-iso-PGF2α level in renal tissue was significantly higher after H/R in group B than that in group A （P 〈 0.01） and the contents of NO and the activity of NOS were significantly lower than those in group A （P 〈 0.01）. The level of 8-iso-PGF2α of group C decreased significantly and the contents of NO and the activity of NOS increased significantly compared with group B and the difference was statistical （P 〈 0.01）. Conclusion： Propofol can decrease levels of 8-iso-PGF2α and increase the contents of NO after H/R injury. It is possibly associated with propofol protecting kidney from injury in rats.

关 键 词：二异丙酚 肾 一氧化氮合酶 一氧化氮 大鼠 

分 类 号：R-332[医药卫生]