冻干红细胞干燥过程及冻干样品的微CT实验分析  

Micro-CT Experimental Analysis on Drying Process of Red Blood Cells During Freeze-drying and Lyophilized Samples

作　　者：肖鑫[1] 陶乐仁[1] 习德成[1] 华泽钊[1] 

机构地区：[1]上海理工大学低温生物医学技术研究所,上海200093

出　　处：《中国医学物理学杂志》2007年第1期12-16,共5页Chinese Journal of Medical Physics

基　　金：上海市重点学科项目(T0503);上海市教育基金会曙光计划(2003);国家自然科学基金项目(50376040)

摘　　要：在红细胞冷冻干燥过程中,利用微CT扫描技术进行样品升华干燥过程的定时扫描观察,并对冻干品进行内部结构扫描分析,同时计算得到各个样品的孔隙率值。对冻干后的样品,采用三步法复水,用血细胞计数板进行检测。实验结果表明,有机溶剂叔丁醇可以加速冻干过程,缩短干燥时间,并且浓度为10%的比5%的显著。在升华过程中,孔隙率呈下降的趋势。最高的孔隙率出现在含有10%叔丁醇的经甘油预处理的保护剂配方中。经过甘油预处理,细胞的恢复率得到了明显的提高,而叔丁醇的加入,导致一定程度上的恢复率下降。In the freeze-drying process of red blood cells, the samples in plastic tube were scanned with Micro-CT scanning technique at fixed time during sublimation, the inner structures of lyophilized samples were scanning analyzed, and the porosities of freeze-drying and lyophilized samples were calculated simultaneously. The dried samples were rehydrated with three steps and evaluted by a haemacytometer. The results are as follows： Organic solvent tea-butyl alcohol （TBA） can accelerate drying process, shorten drying time, and the effect of concentration 10% is more notable than 5% one. During sublimation process, porosity show descending trend. The lyoprotective protocols containing 10% TBA and pretreated by glycerol has the highest porosity. Pretreated by glycerol is profit for cell recovery and TBA may decrease the cell recovery rate to some extent.

关 键 词：冷冻干燥 红细胞 叔丁醇 甘油 微CT 

分 类 号：TQ028.63[化学工程] Q256[生物学—细胞生物学]