脑脊液磷酸化tau蛋白与总tau蛋白比值对散发性Creutzfeldt-Jakob病的诊断价值  

The detection of phosphorylated tau and phospho-tau/total tau ratio in cerebrospinal fluid for the diagnosis of sporadic Creutzfeldt-Jakob disease

作　　者：孙瑞红[1] 林世和[1] 于雪凡[1] 赵节绪[1] 江新梅[1] 宋晓南[1] 

机构地区：[1]吉林大学第一医院神经内科

出　　处：《脑与神经疾病杂志》2007年第1期4-5,29,共3页Journal of Brain and Nervous Diseases

基　　金：国家自然科学基金资助项目(30070266;30470589);黑龙江省科技计划项目(GC04C313)

摘　　要：目的通过对CJD病人脑脊液磷酸化tau蛋白与总tau蛋白比值的研究,探讨其对散发性CJD的诊断价值。方法采用双抗体夹心酶联免疫吸附法(ELISA)检测13例散发性CJD、11例其它痴呆及29例非痴呆脑脊液磷酸化tau蛋白。同时测定脑脊液磷酸化tau蛋白与总tau蛋白的比值。结果1.CJD组与其它两组相比,p-tau/t-tau的比值有明显差异(P<0.05)。2.在CJD组高浓度的磷酸化tau蛋白与进展快,病程短有关(P<0.01)。结论测定p-tau/t-tau的比值有助于CJD与AD等痴呆类疾病的鉴别,对判定预后有一定帮助。Objective：To detect phosphorylated tau and phospho-tau/total tau ratio in the cerebrospinal fluid（CSF） of sporadic Creutzfeldt-Jakob disease and to investigate its diagnostic value. Methods： We analysed cerebrospinal fluid samples 53 patients for phosphorylated tau protein using an enzyme linked immunoassay （ELISA）, and detected phospho-tau/ total tau ratio. Results： 1. The P-tau/total tau ratio was lowest in patients with CJD compared with OD and NDC（P〈0. 05）. The P-tau/total tau ratio discriminated patients with CJD from other dementia. 2. Phosphorylated tau concentration was clinically correlated with a significantly shorter duration （P〈0. 01）. Conclusions： Although the determination of phosphorylated tau levels cannot be used as a diagnostic biomarker, it may prove useful for estimating the prognosisof sCJD. Determination of P-tau/total tau ratio in the CSF is helpful to distinguish CJD from other dementia.

关 键 词：散发性CJD 脑脊液 磷酸化TAU蛋白 

分 类 号：R742.8[医药卫生—神经病学与精神病学]