Propentofylline对体外培养的星形胶质细胞GFAP表达的影响  

Effect of propentofylline on GFAP expression in astrocytes cultured in vitro

作　　者：廖光军[1] 陈安民[1] 郭风劲[1] 周海宇[1] 肖卫东[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院骨外科,湖北武汉430030

出　　处：《中国医师杂志》2007年第1期24-26,共3页Journal of Chinese Physician

摘　　要：目的观察体外培养的星形胶质细胞损伤后，propentofylline对其胶质纤维酸性蛋白（GFAP）表迭的改变，探讨propentofylline对损伤星形胶质细胞的影响。方法建立体外培养大鼠纯化的星形胶质细胞机械损伤模型，利用免疫细胞化学方法观察propentofylline干预后星形胶质细胞GFAP的变化，星形肢质细胞机械损伤后星形胶质细胞随机分为2组（n=2）：药物干预组、对照组。药物干预组子propentofylline处理，用免疫细胞化学染色的方法和图像分析测定法观察星形胶质细胞GFAP蛋白表达的变化及星形胶质细胞的数量。结果机械损伤后12h后损伤边缘区GFAP细胞数目增加、胞体肥大，GFAP表达增强，propentofylline干预后GFAP表达增强被抑制，胞体肥大程度减轻，GFAP阳性细胞数减少。结论propentofylline对损伤后的体外培养的星形胶质细胞的活性具有明显的调节作用。Objective To investigate the effect of propentofylline on GFAP expression in in vitro cultured astrocytes（AS） after mechanical injury. Methods The modal of mechanical injury on in vitro cultured and purified astrocytes （AS） was established. AS were randomly divided into two groups ： group A and group B. Group A was not treated and group B was treated with propentofylline after injury. Immunohistochemical technique and imaging analysis were used to detect the expression of GFAP and the number of GFAP positive cells. Results The expression of GFAP and the number of GFAP positive cells wereincreased after injury. Injured AS had hypertrophy; their proliferation, the expression of GFAP and the number of GFAP -positive cells were increased. After treated with propentofylline, the hypertrophy of AS was attenuated and the expression of GFAP and the number of the GFA P -positive cells were decreased. Conclusion Mechanical injury can promote responsive proliferation. Propentofylline could modulate the hypertrophy and proliferation of AS effectively after mechanical injury.

关 键 词：二羟丙茶碱/药理学 星形细胞 神经胶质原纤维酸性蛋白质 

分 类 号：R74[医药卫生—神经病学与精神病学]