枯草芽孢杆菌JSIM-X-13-4突变株腺苷发酵研究  

Study on the Fermentation of Adenosine by Strain Bacillus subtilis JSIM-X-13-4

作　　者：王红连[1] 张佳俊[2] 赵黎明 杜郭君[1] 张一平[1] 柏建新[1] 邓崇亮[1] 

机构地区：[1]江苏省微生物研究所有限公司 [2]同济大学,上海200092 [3]江南大学,无锡214036

出　　处：《食品与发酵工业》2006年第12期44-47,共4页Food and Fermentation Industries

基　　金：江苏省社会发展资助项目(No.B597080)

摘　　要：对1株黄嘌呤、硫胺素、组氨酸三重缺陷型及8-氮杂鸟嘌呤抗性的腺苷突变株JSIM-X-13-4进行了摇瓶、自控罐3、000 L中型发酵罐试验。试验发现,该菌株培养基成分除酵母膏外,其余与其亲株肌苷产生菌JSIM-1019基本相同。酵母膏浓度为1.6%-1.8%,摇瓶CaCO3量为3%;发酵罐上适宜pH为6.2,风量在18h提高至1∶0.55,溶氧40%。摇瓶、自控罐、中型发酵罐产苷分别为15.51 g/L、18.11 g/L和17.25 g/L。In this study, the adenosine fermentation by the mutant strain B. subtilis JSIM - X- 13 - 4, which was marked with the xanthine, thiamine and histidine tri-auxotroph and 8-azaguanine resistant, was investigated in shake flask, 22 L fermentor, or 3 000L fermentor. Except for the concentration of yeast extract, the components of the culture medium for the mutant was the same as its parent strain, the inosine producing strain B. subtilis JSIM- 1019. The optimal concentration of yeast extract for the mutant was 1.6 % -1.8 %. The optimal concentration of CaCO3 for the shake-flask fermentation was 3 %. The optimal conditions for the fermentation in fermentor were also identified as follow ： pH at 6.2, dissolved oxygen level controlled at 40 96 with the aeration rate gradually increased to 1：0.55vvm after 18-hour cultivation. Under the optimized fermentation conditions, the productivity of the mutant in shake flask, 22 L fermentor, or 3 000L fermentor was 15.51g/L, 18. llg/L, 17.25g/L, respectively.

关 键 词：枯草芽孢杆菌 营养缺陷型 腺苷 

分 类 号：R965[医药卫生—药理学]