GROWTH AND MORPHOLOGY OF POLYMER-ACTIN COMPLEXES  

作　　者：Hyuck Joon Kwon Kazuhiro Shikinaka Akira Kakugo Hidemitsu Furukawa Yoshihito Osada 龚剑萍 

机构地区：[1]Graduate School of Science,Hokkaido University,Sapporo 060-0810,Japan [2]Graduate School of Science,Hokkaido University,Sapporo 060-0810,Japan SORST,JST,Sapporo 060-0810,Japan

出　　处：《Chinese Journal of Polymer Science》2007年第1期47-55,共9页高分子科学（英文版）

基　　金：This research is financially supported by SORST, JST and the Ministry of Education, Science, Sports, and Culture, Japan (Grant-in-Aid of Creative Scientific Research).

摘　　要：F-actins are semi-flexible polyelectrolytes and can be assembled into large polymer-actin complex with polymorphism through electrostatic interaction with polycations. This study investigates the structural phase behavior and the growth of polymer-actin complexes in terms of its longitudinal and lateral sizes. Our results show that formation of polymer-actin complexes is cooperative, and morphology and growth of polymer-actin complexes depend on polycation species and concentrations of polycation and salt in a constant actin concentration. We found that the longitudinal growth and lateral growth of polymer-actin complexes are dominated by different factors. This induces the structural polymorphism of polymer-actin complexes. Major factors to influence the polymorphism of polymer-actin complexes in polyelectrolytc system have been discussed. Our results indicate that the semi-flexible polyelectrolyte nature of F-actins is important for controlling the morphology and growth ofactin architectures in cell.F-actins are semi-flexible polyelectrolytes and can be assembled into large polymer-actin complex with polymorphism through electrostatic interaction with polycations. This study investigates the structural phase behavior and the growth of polymer-actin complexes in terms of its longitudinal and lateral sizes. Our results show that formation of polymer-actin complexes is cooperative, and morphology and growth of polymer-actin complexes depend on polycation species and concentrations of polycation and salt in a constant actin concentration. We found that the longitudinal growth and lateral growth of polymer-actin complexes are dominated by different factors. This induces the structural polymorphism of polymer-actin complexes. Major factors to influence the polymorphism of polymer-actin complexes in polyelectrolytc system have been discussed. Our results indicate that the semi-flexible polyelectrolyte nature of F-actins is important for controlling the morphology and growth ofactin architectures in cell.

关 键 词：F-ACTIN Polymer-actin complex Polycation concentration （Cp） KCl concentration （Cs） Fluorescence microscopy Transmission electron microscopy （TEM） Rod-like polyelectrolyte. 

分 类 号：O631.3[理学—高分子化学]