大鼠颞叶癫痫点燃后海马NR_2A mRNA、NR_2B mRNA的时空表达变化  

Temporal and spatial expressions of NR_2A mRNA and NR_2B mRNA in hippocampus in temporal lobe epileptic rats after kindling

作　　者：梁建民[1] 时颖[2] 崔璐[3] 张淑琴[4] 

机构地区：[1]吉林大学第一医院儿科 [2]武警吉林省总队医院内科,吉林长春130052 [3]吉林大学生物与农业工程学院 [4]吉林大学第一医院神经内科,吉林长春130021

出　　处：《吉林大学学报（医学版）》2007年第1期47-50,共4页Journal of Jilin University：Medicine Edition

基　　金：中国博士后基金资助课题(2005037033);长春市科技发展基金资助课题(176s15)

摘　　要：目的:探讨海马NMDA受体NR2A、NR2B亚单位的时空表达变化与颞叶癫痫后脑损伤的关系。方法:将雄性Wistar大鼠随机分为3组,正常对照组5只,假手术组和海人酸(KA)致痫组各30只,采用KA杏仁核微量注射方法建立经典的大鼠颞叶癫痫点燃模型;假手术组和KA致痫组分别按点燃后6、24、72 h和7、14、21 d时间点分为6组,每组5只。采用原位杂交方法检测海马神经元NR2A mRNA、NR2B mRNA时程表达变化。结果:癫痫组海马各区NR2A mRNA阳性细胞表达率均高于假手术组(P<0.05),于点燃后21 d逐步恢复正常。NR2B mRNA在癫痫组海马DG和CA1区阳性细胞表达率总体上高于假手术组(P<0.05),在CA3区与假手术组比较差异无显著性。回归分析显示NR2A mRNA和NR2B mRNA的阳性细胞表达率与颞叶癫痫存在明显的正相关关系(β=0.154,P=0.0001),NR2B mRNA表达与颠叶癫痫的发生存在正相关关系(β=0.102,P=0.0001)。结论:颞叶癫痫大鼠海马NR2A mRNA、NR2B mRNA存在时空差异性表达改变可能与颞叶癫痫及其脑损伤过程有关。Objective To investigate the relationship between the expression changes of NMDAR subunits in hippocampus and temporal lobe epilepsy. Methods Male Wistar rats were divided into three groups, five were in normal group;the other two groups, sham and epilepsy groups, were divided into 6 subgroups according to the time points of 6, 24 and 72 h, 7, 14, and 21 d after kindling, respectively, each subgroup had 5 rats. Typical kindled model of temporal lobe epilepsy was established by kainic acid （KA） microinjected into right nucleus amygdalae in male Wistar rats. In situ hybridization was used to detect the expression changes of NR2 A mRNA and NR2 B mRNA in hippocampus after KA kindling. Results In general, NR2A mRNA significantly increased in regions of hippocampus in epilepsy group than that in sham group （P〈0. 05）, and gradually returned to normal level in epileptic rats after kindled for 21 d （P〈0.05）. NR2 B mRNA was significantly increased in DG and CA1 regions in epilepsy group than that in sham group （P〈0.05）, while without difference in CA3 region between them. Stepwise regression analysis showed that the expressions of NR2 A mRNA and NR2 B mRNA in hippocampus were positively correlated with temporal lobe epilepsy （P= 0. 0001）. Conclusion NR2 A mRNA and NR2 B mRNA expressions in hippocampus present temporal and spatial changes in epileptic rats, which may be involved in the mechanisms of temporal epilepsy and the brain injury.

关 键 词：癫痫 颞叶 海马 受体 N甲基-D-天冬氨酸 

分 类 号：R742.1[医药卫生—神经病学与精神病学] R-332[医药卫生—临床医学]