rhKD/APP对急性肝损伤小鼠的保护作用  

Hepatoprotective effects of rhKD/APP on acute hepatic injury

作　　者：张馨木[1] 贺巾超[1] 杨莉莉[1] 张赢予[1] 董文[2] 侯立中[1] 颜炜群[1] 

机构地区：[1]吉林大学再生医学科学研究所生物化学研究室,吉林长春130021 [2]天津市红桥医院骨科,天津300130

出　　处：《吉林大学学报（医学版）》2007年第1期51-53,F0002,共4页Journal of Jilin University：Medicine Edition

基　　金：国家高技术研究发展计划(863计划)项目资助课题(2004AA205020)

摘　　要：目的:观察rhKD/APP对四氯化碳(CCl4)所致急性肝损伤的保护作用,探讨其可能的作用机制。方法:采用CCl4建立小鼠急性肝损伤模型,取小鼠60只随机分为6组(每组10只),分别设为空白对照组、模型组、阳性药对照组及rhKD/APP小中大剂量(4.5、9.0、18.0 mg.kg-1)组。测定实验小鼠血清丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)及肝组织丙二醛(MDA)、肝重系数并观察肝脏组织病理学变化。结果:rhKD/APP小、中、大剂量组血清ALT、AST活性和肝组织中MDA低于模型组(P<0.05或P<0.01);肝脏组织病理切片显示,模型组有炎细胞浸润,肝细胞坏死,与模型组相比,rhKD/APP小、中、大剂量组肝组织损伤程度较轻,且随着rhKD/APP剂量增加肝组织损伤程度逐渐减轻。结论:rhKD/APP对CCl4所致急性肝损伤小鼠具有明显的保护作用。Objective To observe the protective effects of rhKD/APP on carbon tetraehloride-indueed acute hepatic injury and find possible mechanisms of hepatic injury. Methods Sixty mice were randomly divided into 6 groups （n：10）： control, model, positive control and rhKD/APP groups with different doses （ 4.5, 9.0, 18.0 mg · kg^-1 ）. Pathologic models of acute hepatic injury in mice caused by carbon tetrachloride were set up and rhKD/APP with different dosage were administered （ip） to mice. Effects of rhKD/APP on hepatic injury were observed by measuring enzyme level （AST and ALT） in serum, hepatic malondialdehyde and morphology of hepatic tissue section . Rosults Compared with model group, serum ALT and AST concentrations in rhKD/APP groups with different doses were decreased significantly （P〈0.01, P〈0.05）, MAD in hepatic tissue in rhKD/APP groups with different doses were decreased （P〈0.01, P〈0.05）. The pathological results showed that there were inflammatory ceils and hepatocellular necrosis in model group, the protective effects of rhKD/APP on hepatic injury showed linear relation to the drug dose.Conclusion rhKD/APP has markedly protective effects on acute hepatic injury induced by carbon tetraehloride.

关 键 词：RHKD/APP 蛋白酶抑制药 肝/损伤 急性病 四氯化碳 

分 类 号：R575.02[医药卫生—消化系统]