食管癌患者手术前后免疫功能改变对外周血细胞凋亡的影响  

Effect of Cellular Apoptosis on Immunologic Function Change of Peripheral Blood between Esophageal Carcinoma Patient before and after Operation

作　　者：林珏龙[1] 谢仰民[1] 沈志忠[2] 朴仲贤[1] 洪超群[3] 杨卫平[3] 

机构地区：[1]汕头大学医学院,广东汕头515031 [2]汕头大学医学院第一附属医院,广东汕头515031 [3]汕头大学医学院肿瘤医院,广东汕头515031

出　　处：《激光生物学报》2007年第1期39-42,共4页Acta Laser Biology Sinica

基　　金：广东省科技厅项目(2006B36001012;2004B60301006)

摘　　要：为探讨食管癌手术前后患者外周血细胞的凋亡情况。利用流式细胞仪对70例食管癌手术前及其手术后10天～14天患者外周血细胞的DNA和CD4，CD8和CD16／56含量进行了分析。结果显示：手术前后患者外周血细胞的凋亡有差异（P〈0．05），但血细胞的DNA合成期细胞比率（S-phage fraction，SPE）、细胞增长指数无明显的差异（P〉0．05）。两组的CD4／CD8比值或CD16／56比例有明显差异（P〈0．001）。实验数据表明：手术前患者的细胞凋亡增加可能有利于平衡肿瘤细胞的恶性增殖；而外周血细胞的CD8、NK（自然杀伤细胞，natural killer cells）增高，可能有助于这种平衡作用。但手术后患者肿瘤负荷减轻，免疫功能恢复常态，细胞凋亡率相应降低。To.study apoptosis of peripheral blood cell of esophageal carcinoma patients. Peripheral blood cell DNA content, CD4, CD8 and CD16/56 count were analyzed with flow cytometry. Blood samples were collected before surgical operation and 10 days - 14 days after operation from 70 patients who had oesophageetomy. The results showed that apoptosis （Ape） was different in peripheral blood cells before and after operation （P〈0.05）, but S-phage fraction （SPF） or proliferation index （PI） was not （P 〉0.05 ）. A significant difference between the groups was noted for CD4/CD8 ratio or the percentage of CD16/56 （P 〈 0. 001 ）. Our data showed that increasing cellular apoptosis before operation might be beneficial in balancing malignant proliferation of tumor cell, and higher level of CD8 and NK might assist this balance in peripheral blood cell, But after operation, tumor load of the patient was alleviated, immunologic function was restored to normal state, and cellular apoptosis was decreased accordingly.

关 键 词：食管癌 外周血细胞 手术 凋亡 流式细胞术 

分 类 号：R735.1[医药卫生—肿瘤]