中枢神经系统感染者血清和脑脊液中NO、NOS动态变化的研究  

A Study of Dynamic Changes of NO、NOS in Serum and CSF in Patients with CNS Infection

作　　者：刘沛[1] 孔繁元[2] 吴若芬[3] 李莉[3] 潘月英[3] 

机构地区：[1]安徽省淮南市第一人民医院神经内科,淮南市232007 [2]宁夏医学院附属医院神经内科,银川750004 [3]宁夏医学院附属医院中心实验室,银川750004

出　　处：《宁夏医学院学报》2007年第1期26-27,共2页Journal of Ningxia Medical College

摘　　要：目的观察中枢神经系统(CNS)感染患者脑脊液(CSF)及血清中一氧化氮(nitric oxide,NO)浓度、总一氧化氮合酶(nitric oxide synthase,NOS)活力的动态变化,为临床辅助治疗中枢神经系统感染采用NO、NO前体或NOS抑制剂提供实验室依据。方法采用硝酸还原酶法和化学比色法检测30例CNS感染患者治疗前、治疗2周后CSF中及入院时、住院第3、59、、14天血清中NO浓度、总NOS活力的动态变化。结果CNS感染患者血清中NO浓度、总NOS活力在入院时及住院第9天明显增高(P<0.05或0.01),其它时间点的变化无统计学意义;治疗前CSF中NO浓度、总NOS活力较对照组及治疗2周后明显增高(P<0.05)。结论说明NO、NOS参与了CNS炎症过程,提示临床采用NO、NOS前体或NOS抑制剂辅助治疗CNS感染在病程的早期和中期将是最合适的。Objective To observe the dynamic changes of NO, NOS in serum and CSF in patients with CNS infection and lay an experimental basis for selecting NO, NO donor or NOS inhibitor to treat CNS infection. Methods With the method of nitrate redutase and the method of chromometry, the dynamic changes of NO、 NOS in serum on admission, on 3,5,9,14 day after admission and in CSF before treatment and two weeks after treatment were measured. Results The concentration of NO in serum and the activation of total NOS of CNS infection patients on admission, on the ninth day after admission significantly increased in CNS infected patients. No difference of the concentration of NO and the activation of total NOS in serum of CNS infection patients on the third day 、 on the fifth day 、 on the fourteenth day after admission was found between CNS infected patients and controls. The concentration of NO in CSF and the activation of total NOS in CSF before treatment in CNS infected patients were significantly higher than that in controls. Conclusions Dynamic changes on the concentration of NO and the vigor of total NOS in serum and CSF in CNS infection patients were found. The dynamic changes of the concentration of NO in serum, the activation of total NOS in serum indicated that deploying NOS inhibitor as assistance treatment for CNS infection was effective during early and middle stage of disease development.

关 键 词：病毒性脑膜炎 细菌性脑膜炎 结核性脑膜炎 一氧化氮 一氧化氮合酶 

分 类 号：R742.9[医药卫生—神经病学与精神病学]