SD大鼠糖尿病创面与正常创面中TGF-β1、TGF-β3的表达差异  

Different TGF-β1 and TGF-β3 expression in the granulation tissue of wound healing between normal and diabetic SD rats

作　　者：李福伦[1] 李斌[1] 王振宜[1] 徐蓉[1] 范斌[1] 徐文彬[1] 金伟[1] 肖秀丽[1] 王一飞[1] 

机构地区：[1]上海中医药大学附属岳阳中西医结合医院皮肤科,上海200437

出　　处：《中国美容医学》2007年第1期35-37,共3页Chinese Journal of Aesthetic Medicine

基　　金：国家自然科学基金资助项目(39900195);上海市重点学科建设项目资助项目(T0304)

摘　　要：目的:通过观察大鼠正常创面与糖尿病模型创面肉芽组织中TGF-β1、TGF-β3不同时段表达水平的差异,探求糖尿病创面“难愈”的可能机制。方法:采用SD大鼠实验性正常创面及糖尿病创面背部全层皮肤缺损模型,分别于不同时段(3天、7天、11天)取材,EnVision二步法免疫组化测定,并行图像扫描定量分析TGF-β1、TGF-β3水平变化。结果:创伤后3天、7天和11天,正常创面模型组TGF-β1表达分别为(25.56±6.86)、(24.79±5.62)和(28.57±6.39),TGF-β3表达分别为(39.69±5.46)、(23.20±2.45)、(13.69±3.63)。而糖尿病溃疡模型组TGF-β1表达分别为(7.10±3.74)、(9.47±1.72)和(7.87±2.53),TGF-β3表达分别为(14.99±1.87)、(18.72±5.01)和(8.68±3.39)。糖尿病性创面肉芽组织中各时段(3天、7天和11天)TGF-β1及TGF-β3表达水平均低于正常创面组(P<0.05)。结论:SD大鼠糖尿病创面TGF-β1及TGF-β3的低水平表达,可能是创面愈合迟缓的原因之一。Objective To study the different TGF-β1 and TGF-β3 expression of the granulation tissue between normal and diabetic SD rats in different wound healing periods. Methods After the establishment of full thickness dermal wounds of 2cm diameter inflicted on the SD rats back, the specimens were collected from the wound edge on the third.seventh and eleventh days after injury respectively. The specimens were subjected to immunohistochemical methods and image analysis of TGF-β1 and TGF-β3. Results On the third.seventh and eleventh day of wound healing respectively, TGF-β1 and TGF-β3 expressions in the granulation tissue of diabetic rats were much lower than those of the normal rats. Conclusion The lower level expressions of TGF-β1 and TGF-β3 in diabetic ulcer may be one cause of the difficulty wound healing.

关 键 词：糖尿病 创面愈合 TGFΒ 

分 类 号：R641[医药卫生—外科学]