磺胺类药物在动物性食品中的残留与检测  

Sulfonamide Residues and Detection in Animal Food

作　　者：鲁晓翠[1] 侯玉泽[1] 邓瑞广[2] 彩鸿翔[1] 王自良[3] 柴书军[2] 张改平[2] 

机构地区：[1]河南科技大学食品与生物工程学院,河南洛阳471003 [2]河南省农业科学院,河南郑州450002 [3]河南科技学院动物科学系,河南新乡453003

出　　处：《动物医学进展》2007年第2期70-74,共5页Progress In Veterinary Medicine

基　　金："十五"国家重大科技专项"食品安全关键技术综合示范"(2001BA804A30-08)

摘　　要：磺胺类药物是一类广谱抗菌药,临床上主要用于预防和治疗细菌感染性疾病,还常作为饲料添加剂在动物生产中长期应用。然而,磺胺药会引起人过敏性反应,且可能有致癌性。因此,磺胺类药物的不合理使用,使其在动物性食品中残留引起生态环境污染和人类健康危害的潜在威胁已倍受关注。因此,研究出一种操作简便、快速、灵敏的磺胺类药物残留检测方法,对于畜产品的安全具有实际意义。酶联免疫吸附法(ELISA)作为一种免疫学检测技术不仅具有上述优点,而且准确性好、特异性强、检测成本低,还可用于大批量样品的检测。而单抗在试剂的标准化及抗体的大量供应上明显优于多抗,所以单克隆抗体技术的应用在药残检测上是主要的发展方向。Sulfonamides are antibiotics which have wide antimicrobial spectrum. They are used to prevent and cure bacterial infections and are added into forage to feed animals. But sulfonamides can cause anaphylactic reaction and have carcinogenicity. So they have been more and more attached importance to the hurt to people and the pollution to environment by sulfonamide residues for its unreasonable utilization. Therefore, it is acturally significant to study sulfonamide detection methods in animal product security. Enzyme linked immunosorbent assay is convenient, fast, sensitive and has good accuration,strong specificity. The detection price is low and can detect a large number of samples. And Monoclone antibodies surpass polyclone antibodies on reagent standardization and supply. So monoclone antibody technology is the main development in drug residue detection.

关 键 词：磺胺 动物性食品 残留 免疫学 检测 

分 类 号：S859.84[农业科学—临床兽医学] S859.79[农业科学—兽医学]