NF-κB、COX-2在结肠癌及癌前病变中的表达及其临床意义  

Expressions of NF-κB and COX-2 in colon precancerous and cancerous lesions:their correlation with clinical features

作　　者：刘莹[1] 崔涛[1] 朱祖安[2] 费素娟[3] 杨雪梅[1] 

机构地区：[1]徐州医学院病理学教研室,江苏徐州21002 [2]徐州市中心医院放疗科,江苏徐州221002 [3]徐州医学院附属医院消化内科,江苏徐州221006

出　　处：《徐州医学院学报》2007年第1期15-19,共5页Acta Academiae Medicinae Xuzhou

基　　金：江苏省高校自然科学基金(05KJD320234)

摘　　要：目的探讨核转录因子-κB(NF-κB)及环氧合酶-2(COX-2)在结肠癌及癌前病变的表达及其与临床特征之间的关系。方法通过免疫组织化学染色(PowerVisionTM两步法)法检测15例溃疡性结肠炎、14例绒毛状腺瘤、17例重度不典型增生及74例结肠癌组织标本中NF-κB、COX-2的表达情况。结果COX-2在正常黏膜、溃疡性结肠炎、绒毛状腺瘤、重度不典型增生、结肠癌中阳性表达率分别为20.00%(2/10),46.67%(7/15),35.71%(5/14),47.06%(8/17),64.47%(49/76);NF-κB在正常黏膜、溃疡性结肠炎、绒毛状腺瘤、重度不典型增生、结肠癌中阳性表达率分别为10.00%(1/10),53.33%(8/15),21.43%(3/14),47.06%(8/17),57.89%(44/76);COX-2、NF-κB在结肠癌中的表达与肿瘤大小、肿瘤分期相关,与性别、年龄无关;在结肠癌肿瘤组织中,NF-κB与COX-2表达具有相关性(χ2=5.0552,P=0.025)。结论在炎症情况下,NF-κB持续表达可能导致COX-2过度表达,引起肠黏膜细胞过度增生,成为结肠癌形成的早期事件并在结肠癌的浸润和转移中起到重要作用,因而提示NF-κB可作为肿瘤防治新的靶点。Objective To explore the expressions of nuclear transcription factor - κB （ NF - κB） and cyclooxygenase -2 （COX -2） in colon precancerous and cancerous lesions and define the correlation of NF- κB and COX -2 expressions with the clinical features. Methods The expressions of NF- κB and COX -2 in colon precancerous and cancerous lesions were determined by immunohistochemistry （Power VisionTM two step method） in 15 cases of ulcerative colitis, 14 villous adenoma, 17 colon high atypical hyperplasia and 74 colon carcinoma. Results NF - κB and COX -2 expressions were significantly higher in colon carcinoma than in ulcerative colitis, villous adenoma or colon atypical hyperplasia. [ The expression of COX -2 in normal colon mucosa, ulcerative colitis, villous adenoma, high atypical hyperplasia and colon carcinoma was 20.00% ,46.67% ,35.71% ,47.06% ,and 64.47%, respectively; that of NF - κB was 10.00%, 53.33% , 21.43% , 47.06% , and 57.89%, respectively, The over expression of NF - κB and COX - 2 in colon carcinoma was related to the size and Duke＇ s stage of the tumor （ P 〈 0.05 ）, but not to the patient＇ s age or sex. The expression of NF - κB in colon carcinoma was positively correlated with COX - 2 expression （ X^2 = 5. 0552, P =0. 025 ） ]. Condusion In an inflammatory process, the over expression of NF - κB will enhance the expression of COX - 2 and lead to hyperplasia in colon mucosa. As this is the early event of colon carcinogenesis and plays important roles in the infiltration and metastasis of the cancer, NF - κB could be taken as a new target in the efforts to be made for colon cancer prevention and treatment.

关 键 词：核转录因子-ΚB 环氧合酶-2 溃疡性结肠炎 绒毛状腺瘤 结肠癌 

分 类 号：R735.35[医药卫生—肿瘤]