纳米硒对断奶仔猪肝脏谷胱甘肽过氧化物酶和脱碘酶I活性的影响  

Effect of Nano-Selenium on the Activities of Glutathione Peroxidase And Type-I Deiodinase in the Liver of Weanling Pigs

作　　者：张红梅[1] 夏枚生[2] 胡彩虹[1] 

机构地区：[1]浙江大学动物科学学院动物分子营养学教育部重点实验室,杭州310029 [2]浙江大学理学院,杭州310027

出　　处：《生物医学工程学杂志》2007年第1期153-156,共4页Journal of Biomedical Engineering

基　　金：国际科学基金资助项目(B/3452-1);宁波市科技计划项目(2005C100120)

摘　　要：研究了纳米硒和亚硒酸钠对断奶仔猪肝脏谷胱甘肽过氧化物酶和脱碘酶Ⅰ活性的影响。将纳米硒和亚硒酸钠2种硒源分别以0．1、0．2、0．3、0．4、0．5、1．0mg／kg6个硒水平添加到基础日粮中，配制成12种试验日粮，基础日粮作对照，饲养“杜长大”断奶仔猪（8．3kg左右）。结果表明：以亚硒酸钠形式添加的硒浓度为1．0mg／kg时，仔猪生长性能和GSH-Px活性显著低于0．2～0．4mg／kg硒添加水平（P〈0．05），纳米硒添加浓度为1．0mg／kg，仔猪生长性能和GSH-Px活性仍然保持在高峰平台；硒源添加浓度为0．10～0．40mg／kg时，两种硒源对GSH-Px活性的影响无显著差异（P〉0．05）；在0．50和1．0mg／kg硒添加水平上，纳米硒组GSH-Px活性显著高于亚硒酸钠组（P〈0．05）。基础日粮组肝脏脱碘酶Ⅰ活性显著低于各硒源的添加水平（P〈0．05），硒源和硒添加水平对脱碘酶Ⅰ活性没有显著影响（P〉0．05）。上述结果提示：纳米硒的Weinberg剂量一效应的最适剂量范围宽于亚硒酸钠。To study the effects of nano elemental selenium （Nano-Se） or sodium selenite （Na2SeO3） on the activities of glutathione peroxidase （GSH-Px） and Type-Ⅰ deiodinase in the liver. A total of 234 weanling pigs （Duroc×Landrace×Yorkshire） at an average initial body weight of 8.3 kg were allocated to 13 treatments. The thirteen dietary treatments were basal diet only （containing 0.04 mg/kg Se）, basal diet＋0.1.0.2,0.3.0.4.0.5. 1.0 mg/kg Se as Na2SeO3 or Nano-Se, respectively. The results were as follows ： Supplementation with 1.0 mg/ kg Se as Na2SeO3 reduced （P〈0.05） growth performance and GSH-Px activities as compared with the addition of a concentration range of 0.20-0.40 mg/kg Se. When Nano-Se was added to the diet, the growth and GSH-Px activities remained steady at the peak value as at a concentration of 1.0 mg/kg Se; There were no difference in the activities of GSH-Px between the treatments of Nano-Se and Na2SeO3 when added concentration of Se was 0.10- 0. 40 mg/kg. The pigs had higher （P〈0. 05） activities of GSH-Px at a concentration range of 0. 50 and 1.0 mg/kg as Nano-Se than Na2SeO3; Supplentation with Se increased the activity of Type-Ⅰ deiodinase in liver, however, the increased extent was affected by neither Se sources nor added concentration of Se. The results implicated that for the best concentration range of Weinberg curve, Nano-Se is wider than Na2SeO3.

关 键 词：硒 纳米 谷胱甘肽过氧化物酶 脱碘酶Ⅰ 仔猪 

分 类 号：R151[医药卫生—营养与食品卫生学]