DC/C6融合瘤苗防治C6胶质瘤的实验研究  

Experimental study of antitumor immunity of DC/C6 fusion vaccine

作　　者：王东海[1] 李新钢[2] 王英 曲迅[4] 李刚[2] 宫崧峰[1] 刘泉萌[1] 鲍修风[2] 

机构地区：[1]山东大学齐鲁医院神经外科实验室 [2]山东大学齐鲁医院神经外科 [3]山东龙口矿业集团中心医院,山东济南250012 [4]山东大学齐鲁医院临床实验中心

出　　处：《中国病理生理杂志》2007年第2期254-257,共4页Chinese Journal of Pathophysiology

基　　金：山东省自然科学基金资助项目(No.2000BB1CJA13);山东省医药卫生系统杰出学科带头人资助项目

摘　　要：目的:探讨DC/C6融合瘤苗防治C6胶质瘤的疗效及作用机制。方法:采用PEG化学融合方法制备融合瘤苗,应用GFAP-FITC免疫荧光检查进行瘤苗的鉴定;立体定向制备大鼠颅内C6肿瘤模型,于种瘤后5 d经尾静脉注射107融合瘤细胞、107DC以及100μL PBS,分设为A、B、C 3组,采用Log-rank对数秩检验进行生存分析,并行肿瘤标本HE染色及抗CD8M cab免疫组化染色。结果:融合瘤苗GFAP-FITC免疫荧光检查阳性;Log-rank生存分析对数据进行对数秩检验,结果表明A组与B、C组进行比较均有统计学意义(P<0.01);A组晚期死亡大鼠(>31 d)HE染色见较多的炎性细胞浸润,CD8M cab免疫组化染色阳性。结论:DC/C6融合瘤苗能够有效的发挥抗原提呈、活化T淋巴细胞的功能,CD8+T细胞参与抗胶质瘤免疫反应。AIM： To study the anti - tumor effect and mechanism of fusion vaccine on DCs and C6 glioma cells. METHODS： PEG was used to fuse DCs with C6 glioma cells. Immunofluorescence with GFAP - FITC was used to identify the DC/C6 fusion cells. Rat brain glioma models were made by stereotactic technique. After 5 days of inoculation of C6, 10^7 fusion cells were injected through tail vein in group A. The same number of DCs and the same volume of PBS were used in group B and group C. The survival time of rats in these three groups was analyzed by Log - rank survival analysis. Tumor samples were checked by HE staining and immunohistochemical staining with CD8Mcab. RESULTS： Positive resuh of GFAP - FITC immunofluorescence was observed in DC/C6 fusion cells. The Log - rank survival analysis showed that statistically significant difference in group A was observed compared to that in group B and group C （ P 〈 0. 01 ）. Tumor sample stained with HE showed that many inflammatory cells infiltrated in tumor tissues. The result of immunohistochemical staining with CD8Mcab was positive. CONCLUSION： DC/C6 fusion cells had the ability of antigen presentation and activating T lymphocytes effectively. CD8^＋T lymphocytes play an important role in antitumor immunity.

关 键 词：树突细胞 神经胶质瘤 瘤苗 免疫疗法 

分 类 号：R363[医药卫生—病理学]