硝普钠诱导被动致敏的人气道平滑肌细胞凋亡  

Effect of sodium nitroprusside on apoptosis in human airway smooth muscle cells of passive sensitization by serum from allergic asthmatic patients

作　　者：叶涛[1] 徐永健[1] 张珍祥[1] 刘先胜[1] 李亚清[1] 唐以军[1] 钟声[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院呼吸病研究室,湖北武汉430030

出　　处：《中国病理生理杂志》2007年第2期276-279,共4页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.30270583)

摘　　要：目的:研究一氧化氮(NO)供体硝普钠(SNP)是否诱导被动致敏的人气道平滑肌细胞(HASMCs)凋亡。方法:将人气道平滑肌细胞用哮喘患者血清被动致敏后,用SNP(NO的供体)干预,通过四甲基偶氮唑盐(MTT)法测定细胞生长率的变化,用原位末端标记法和流式细胞术检测SNP对被动致敏的人气道平滑肌细胞凋亡的影响。结果:(1)MTT法测定:SNP+哮喘血清致敏组的细胞存活率明显低于哮喘血清致敏组细胞存活率(n=5,P<0.05)。(2)TUNEL(原位末端标记)法检测:SNP干预的被动致敏的HASMC组的凋亡指数明显高于哮喘血清致敏组(n=4,P<0.01)。(3)流式细胞术测定SNP+哮喘血清致敏组的HASMCs凋亡率明显高于哮喘血清致敏组(n=4,P<0.05)。结论:SNP能抑制被动致敏的HASMCs的增殖,并且能诱导被动致敏的HASMCs凋亡,这种作用可能与治疗哮喘患者的气道重建有关。AIM： To investigate NO - donor sodium nitroprusside （SNP） - induced apoptosis of human airway smooth muscle cells （HASMCs） of passive sensitization by serum from allergic asthmatic patients. METHODS ： The technique of human airway smooth muscle （HASMCs） passively sensitized with serum from allergic asthmatic patients was adopted. The effect of SNP on the survival rate of passively sensitized HASMCs was detected by MTT method. Apoptosis of cells was detected by TUNEL and flow cytometry. RESULTS： （ 1 ） Compared to sensitized group, the survival rate of passively sensitized HASMCs decreased in SNP ＋ sensitized group （ n = 5, P 〈 0. 05 ）. （2） TUNEL showed that apoptosis index of passively sensitized HASMCs was significantly increased following SNP treatment （n =4, P 〈0.01 ）. （3） Flow cytometry showed that apoptosis rate of passively sensitized HASMCs was significantly increased following SNP treatment （ n = 4, P 〈0. 05）. CONCLUSION： SNP inhibits the proliferation of passively sensitized HASMCs and induces apoptosis of passively sensitized HASMCs, which may be related to treatment of airway remodeling in asthma.

关 键 词：一氧化氮 被动致敏 细胞凋亡 肌 平滑 气道 

分 类 号：R363[医药卫生—病理学]