L-精氨酸对肺缺血/再灌注损伤时bcl-2、bax基因表达的影响  

Effect of L-arginine on expression of bcl-2 and bax mRNA in pulmonary injury induced by ischemia-reperfusion in rabbits

作　　者：王万铁[1] 戴雍月[1] 许益笑[1] 邱晓晓 汪洋[1] 郝卯林[1] 倪世容[1] 王方岩[1] 金可可[1] 王卫[1] 郑绿珍[1] 宋张娟[1] 王青[1] 

机构地区：[1]温州医学院病理生理学教研室,浙江温州325053

出　　处：《中国病理生理杂志》2007年第2期288-292,共5页Chinese Journal of Pathophysiology

基　　金：浙江省教育厅科研基金资助项目(No.20000670);温州市科技计划重点资助项目(No.Y2005A080)

摘　　要：目的:探讨L-精氨酸对肺缺血-再灌注损伤(PIR I)时bcl-2、bax基因表达的影响。方法:采用在体兔单肺原位缺血-再灌注模型。实验兔36只,随机分为假手术对照组(sham,12只)、肺缺血-再灌注组(I/R,12只)和肺缺血-再灌注加L-精氨酸组(L-Arg,12只)。分别于再灌注5 h取左肺组织,观察bcl-2、baxmRNA定位表达、凋亡指数(AI)、肺组织湿干重比(W/D)、肺损伤组织学定量评价指标(IQA)及光镜、电镜下的组织形态学改变。结果:在肺小动脉内(外)膜、肺小静脉内膜、肺泡上皮及细支气管上皮,L-Arg组bcl-2 mRNA的表达及bcl-2/baxmRNA的比值显著高于I/R组(均P<0.01),baxmRNA的表达明显低于I/R组(P<0.01);AI、W/D和IQA值显著低于I/R组(P<0.01和P<0.05);肺组织形态学异常改变不同程度减轻。结论:L-精氨酸可上调肺组织bcl-2 mRNA的表达、下调肺组织baxmRNA的表达、调控bcl-2/baxmRNA之间的平衡而减轻细胞凋亡,对PIR I发挥积极的防治作用。AIM ： To investigate the effect of L - arginine （ L - Arg） on expression of bcl - 2, bax mRNA during pulmonary ischemia and reperfusion injury （PIRI） in rabbits. METHODS： Single lung ischemia and reperfusion animal model was used in vivo. The rabbits were randomly divided into three groups： sham operated group （sham, n = 12）, ischemia - reperfusion group （ I/R, n = 12） and I/R ＋ L - arginine group （ L - Arg, n = 12）. Changes of several parameters, which included apoptotic index （AI）, wet to dry ratio of lung tissue weight （W/D） and index of quantitative assessment of histologic lung injury （ IQA）, were measured at 300 min after reperfusion in lung tissue. Meanwhile the location and expression of bcl - 2, bax mRNA as well as the ratio of bcl - 2 mRNA/bax mRNA were observed. The lung tissue was prepared for light microscopic and electron microscopic observation at 60, 180 and 300 min after reperfusion. RESULTS ： As compared with I/R group, in intima and extima of small pulmonary artery, alveoli, and bronchiole epithelia, the expression of bcl - 2 mRNA and the ratio of bcl - 2 mRNA/bax mRNA were increased, and the expression of bax mRNA was decreased in L - Arg treatment group. The values of AI, W/D and IQA showed significantly lower than that in I/R group at 180 minutes after reperfusion in lung tissue （P 〈0. 01 and P 〈0. 05）. Meanwhile, abnormal changes of the lung tissue in morphologically were markedly lessened in L - Arg treatment group. CONCLUSION： L - arginine produces a notable protective effect on PIRI in rabbits by up - regulating bcl - 2 mRNA expression, down - regulating bax mRNA expression in lung tissue and regulating the balance of bcl -2 mRNA and bax mRNA to decrease apoptosis.

关 键 词：再灌注损伤 肺 基因 BCL-2 基因 bax 细胞凋亡 精氨酸 

分 类 号：R363.2[医药卫生—病理学]