血管紧张素Ⅱ对THP-1源性泡沫细胞ATP结合盒转运子A1的影响  

Effects of angiotensin Ⅱ on ATP binding cassette transporter A1 in THP-1 derived foam cells

作　　者：陈志坚[1] 王彦富[1] 廖玉华[1] 梅春丽[1] 彭红玉[1] 郭和平[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院心内科,湖北武汉430022

出　　处：《中国病理生理杂志》2007年第2期316-320,共5页Chinese Journal of Pathophysiology

摘　　要：目的:以THP-1源性泡沫细胞为研究对象,探讨血管紧张素Ⅱ(AngⅡ)对THP-1源性泡沫细胞ATP结合盒转运子A1(ABCA1)表达、细胞内胆固醇含量及胆固醇流出的影响。方法:运用逆转录-多聚酶链反应(RT-PCR)和W estern b lotting分别检测AngⅡ对ABCA1 mRNA与ABCA1蛋白表达的影响,采用酶法,通过荧光分光光度计检测细胞内胆固醇含量,应用液体闪烁计数仪检测胆固醇流出的变化。结果:AngⅡ能引起THP-1源性泡沫细胞胆固醇含量显著升高(P<0.05)、ABCA1表达显著减少(P<0.05),AngⅡ受体拮抗剂厄贝沙坦(Irb)能显著减少细胞内胆固醇含量(P<0.05)、促进细胞内胆固醇流出及减轻AngⅡ对ABCA1的抑制作用(P<0.05)。结论:AngⅡ有通过其受体抑制ABCA1表达,促进泡沫细胞形成,加速动脉粥样硬化的作用。AIM ： To study the influence of angiotensin Ⅱ （ Ang Ⅱ ） on ATP - binding cassette transporter A1 in THP - 1 derived foam cells. The variance of the expression of ABCA1, the content and the effluent rate of cholesterol were also investigated. METHODS： The regulatory effect of Ang Ⅱ on the expression of ABCA1 mRNA and protein in THP - 1 derived form cells were measured by RT - PCR and Western blotting. The effect of variance of cholesterol content was measured by zymechemistry via - fluorospectrophotometer, cholesterol effluent was measured by liquid scintillator. RESULTS ： A positive facilitative effect of Ang Ⅱ on form ceils was observed. Total cholesterol content were increased significantly by Ang Ⅱ treatment （ P 〈 0. 05）. The mRNA and protein of ABCA1 were down - regulated significantly by Ang Ⅱ stimulation （ P 〈 0. 05）. Irbesartan reduced the total cholesterol content significantly （ P 〈 0. 05 ）. Meanwhile, the increase in the effluent rate of cholesterol and the expression of ABCA1 were observed （ P 〈 0. 05 ）. CONCLUSION ： The effects of Ang Ⅱ on the formation of foam cells and atherosclerosis may be correlated to the activation of AT1 receptor and down - regulation of ABCA1.

关 键 词：血管紧张素Ⅱ ATP结合匣式转运子 动脉硬化 泡沫细胞 

分 类 号：R363[医药卫生—病理学]