CCK-8对LPS攻击小鼠IL-1β、IL-6、IL-4、IL-10表达的影响  

Effects of cholecystokinin octapeptide on expression of IL-1β,IL-6,IL-4 and IL-10 in lipopolysaccharide-attacked mice

作　　者：倪志宇[1] 丛斌[1] 李淑瑾[1] 马春玲[1] 闫玉仙[1] 徐锦荣[1] 张国忠[1] 

机构地区：[1]河北医科大学法医学系,河北石家庄050017

出　　处：《中国病理生理杂志》2007年第2期331-335,共5页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.30270529);河北省博士基金资助项目(No.06547008D-5)

摘　　要：目的:观察LPS攻击小鼠IL-1β、IL-6、IL-4、IL-10的动态变化规律及八肽胆囊收缩素(CCK-8)对其表达的影响。方法:将小鼠分为4组:对照组、LPS组(腹腔注射LPS)、LPS+CCK-8组(注射LPS前30 min腹腔注射CCK-8)及CCK-8组(单独注射CCK-8)。用ELISA及PT-PCR方法检测各组小鼠血清、肺组织中IL-1β、IL-6、IL-4、IL-10的含量及mRNA的表达情况。结果:LPS攻击可使小鼠血清及肺组织中IL-1β、IL-6、IL-4、IL-10蛋白及mRNA的表达增加,预先注入CCK-8可显著抑制IL-1β、IL-6的表达,并使IL-4、IL-10的表达进一步增加。结论:CCK-8可能通过抑制LPS攻击小鼠IL-1β、IL-6的表达和进一步增加IL-4、IL-10的表达参与抗炎反应过程,从而减轻LPS引起的肺组织炎症反应。AIM： To observe the effects of cholecystokinin octapeptide （ CCK - 8 ） on the expression of proinflammatory cytokines IL - 1β, IL - 6 and anti - inflammatory cytokines IL - 10, IL - 4 in LPS - attacked mice. METHODS： Kunming mice were randomly assigned and injected intraperitoneally with LPS alone or/and CCK - 8 at different time points. The expression of IL- 1β, IL-6, IL- 10 and IL-4 in the serum and lung tissues were assayed by ELISA and RT -PCR. RESULTS： The expression of IL - 1β, IL -6, IL - 10 and IL -4 were upregulated in LPS - attacked mice. Pre -treatment of CCK -8 decreased both IL- 1β and IL-6 expression and augmented IL- 10 and IL-4 expres- sion in LPS -attacked mice. CONCLUSIONS： CCK- 8 exerts an anti -inflammatory effect by inhibiting the expression of IL - 1β, IL -6 and increasing the expression of IL - 10, IL -4 in LPS - attacked mice, which could alleviate the inflammatory response in lung tissue.

关 键 词：缩胆囊素 脂多糖类 白细胞介素类 

分 类 号：R363[医药卫生—病理学]