抗人CD134单抗对外周血单个核细胞穿孔素mRNA表达的影响  

Effect of anti-human CD134 monoclonal antibody on expression of perforin mRNA in peripheral blood mononuclear cells

作　　者：李鸣[1] 张源潮[1] 

机构地区：[1]山东大学山东省立医院风湿免疫科,山东济南250021

出　　处：《中国病理生理杂志》2007年第2期340-344,共5页Chinese Journal of Pathophysiology

基　　金：山东省科技发展计划资助项目(No.200328)

摘　　要：目的:观察不同浓度抗人CD134单抗对外周血单个核细胞穿孔素mRNA表达的影响及时间效应,旨在探讨抗人CD134单抗延长T细胞存活的可能机制。方法:应用反转录-聚合酶链反应(RT-PCR)技术分别检测不同浓度(1 mg/L、5 mg/L、10 mg/L)抗人CD134单抗对外周血单个核细胞穿孔素mRNA表达的影响,同时检测每种浓度作用不同时间(6 h、12 h、24 h、48 h)对外周血单个核细胞穿孔素mRNA表达的影响。结果:不同浓度CD134单抗作用不同时间,对人外周血单个核细胞穿孔素mRNA表达均有不同程度抑制作用,在24 h该作用达高峰。当CD134单抗浓度>5 mg/L时,这种抑制作用不再增强(P>0.05)。结论:抗人CD134单抗可在转录水平抑制穿孔素表达,该效应在24 h达高峰且会饱和,此乃抗人CD134单抗延长T细胞存活的主要机制。AIM： To study the concentration -response and time course of perforin mRNA in peripheral blood mononuclear cells, as well as mechanism for improving survival of T cells with anti - human CD134 monoclonal antibody. METHODS： The expression of perforin mRNA in peripheral blood mononuclear cell under the effect of various concentrations of anti - human CD134 monoclonal antibody （ 1 mg/L, 5 mg/L, 10 mg/L） and under the effect of any concentration of anti-human CD134 monoclonal antibody for different times （6 h, 12 h, 24 h, 48 h） was determined by reverse transcription -polymerase chain reaction. RESULTS： Various concentrations of anti -human CD134 monoclonal antibody had inhibitory effects on expression of perforin mRNA in peripheral blood mononuclear cells for different times. The expression of perforin mRNA reached its maximal on 24 h at all concentration tested. The inhibitory effects reached a plateau when the concentration of anti - human CD134 monoclonal antibody exceeding 5 mg/L （ P 〉 0. 05 ）. CONCLUSION： Anti - human CD134 monoclonal antibody inhibits perforin expression at transcription level and this effect reaches its maximal and to be satiable at 24 h, which may be the reason for anti -human CD134 monoclonal antibody to improve survival of T cells.

关 键 词：CD134 穿孔素 白细胞 单核 

分 类 号：R363[医药卫生—病理学]