重庆地区抑郁症患者博尔纳病病毒感染的分子生物学研究(英文)  

Molecular biological research on Borna disease virus infection in depressive patients of Chongqing

作　　者：赵立波[1] 谢鹏[1] 牟君[1] 李亚军[1] 邹德智[1] 刘庆军[1] 周锡国 杨建 

机构地区：[1]重庆医科大学附属第一医院神经内科重庆市神经病学重点实验室,重庆400016 [2]重庆市南岸区精神卫生中心 [3]重庆市巴南区精神卫生中心

出　　处：《中国神经精神疾病杂志》2007年第1期18-22,共5页Chinese Journal of Nervous and Mental Diseases

基　　金：国家自然科学基金资助项目(编号:30470605)~~

摘　　要：背景博尔纳病病毒(Borna disease virus,BDV)是一种高度嗜神经的RNA病毒,是人畜共患病博尔纳病(Borna disease,BD)的病原体,可引起从鸟到灵长类的多种动物的中枢神经系统感染[1],表现为以中枢神经系统功能障碍为特征的BD。近年研究发现BDV感染与一些神经精神疾病的发病有关,尤其是精神疾病。但有关BDV感染与抑郁症(depressive disorder,DD)发病之间的关系,目前国内外研究尚有争议。本研究从分子生物学角度进一步探讨BDV与DD发病之间的关系。方法采用巢式逆转录酶聚合酶链反应(nRT-PCR)结合荧光定量聚合酶链反应(FQ-PCR)检测了60例DD患者和120名健康人外周血单个核细胞(PBMCs)中BDV p24基因片段,对FQ-PCR阳性产物进行克隆和基因序列测定,测序结果与人和动物来源的BDV分离株以及标准株Strain V和He/80进行序列比较。对两组阳性率进行Fisher精确概率检验。结果DD组BDV p24基因片段阳性率为5%(3/60);健康组阳性率为0%(0/120)。DD组阳性率高于健康组,差异有显著性意义(P<0·05)。测序结果为5′-CCCTCCAAGTGGAAACCATCCAGACAGCTCAGCGGTGCGACCACTCCGACAGCATCAGGATTCTTGGCGAGAACATCAA-GATACTG-3′。登陆美国国家生物技术信息中心,证实所获得目的基因片段确系BDV p24基因片断,与人类基因组片段和其他病毒基因组片段无同源性。其与马源的BDV病毒株H1766序列比较亲缘关系最近,同源性为97.68%,在2个位点出现突变(nt1675T→C,nt1678C→T)。与其他国际公认的标准病毒株Strain V和He/80比较,同源性分别为96·51%和95·.35%,碱基互换中局限于T-C、和A→G两种。结论中国的DD患者中存在BDV感染,重庆地区DD的发病可能与BDV感染有关。Background Evidences, from recent studies, suggested that Borna disease virus （BDV） infection might be associated with human neuropsychosis, especially psychiatric disorders including depressive disorder（DD）. However, controversy existed about the association between BDV infection and pathogenesis of DD. This study was to explore further whether the infection of Borna disease virus （BDV） is associated with the pathogenesis of depressive disorder （DD）. Methods The p 24 fragment of BDV RNA in peripheral blood mononuclear cells （PBMCs） from 60 DD patients and 120 healthy volunteers was detected by nested reverse transcriptase polymerase chain reaction（nRT-PCR） combined with fluorescence quantitative polymerase chain reaction （FQPCR）. Positive products were cloned and sequenced before being compared with Strain V and strain He/80, from humans and animals. Results The positive rate （5%, 3/60） of BDV p 24 in PBMCs from the DD patients was significantly higher than that （0%, 0/120） from healthy volunteers （ P 〈0.05）. The gene sequence for the posi- tive products showed BDV p 24 in PBMCs from DD patients in Chongqing was most homophylic with H1766 strain detected from ill horses （97. 68%）, with 2 situs mutations （nt 16？5 T→C, nt 16？8 C→ T）, and also similar to the standard strain V（96. 51%）and He/ 80（95. 35 %）, with basic exchanges limited to T- C and A→G. Conclusions There was BDV infection in the DD patients in China, which indicated that the pathogenesis of DD in human beings in Chongqing might be associated with the infection of BDV.

关 键 词：抑郁症 博尔纳病病毒 荧光定量聚合酶链反应 重庆 

分 类 号：R749.4[医药卫生—神经病学与精神病学]