小儿恶性肿瘤四氮唑盐法药敏试验的建立  

CHEMOSENSITIVITY TEST FOR CHILDHOOD TUMORS BY MTT ASSAY

作　　者：郑修军[1] 董蒨[1] 杨传民[1] 江布先[1] 黄婉芬[1] 

机构地区：[1]青岛医学院附属医院儿外科,青岛266003

出　　处：《青岛医学院学报》1996年第4期293-296,共4页Acta Academiae Medicinae Qingdao Universitatis

基　　金：山东省教委科研基金资助课题(批准号:J95K07)

摘　　要：①目的筛选对个体恶性肿瘤病儿敏感的化疗药物,并探讨其最适条件。②方法用四氮唑盐(MTT)法药敏试验对5例恶性肿瘤病儿进行药物筛选。③结果每孔细胞数在5X10^4~5X10^5,培养48~72h,MTT浓度为2.0~2.5g/l时比较适宜。从不同化疗药物对肿瘤细胞的杀伤作用可以看出,不同药物对同一肿瘤细胞的杀伤作用有很大不同,同一药物对同一类型的不同个体肿瘤的杀伤作用也不相同。④结论MTT法药敏试验操作简单、快速,易于推广,可广泛应用于临床,以提高疗效,避免不必要的化疗副作用。Objective To select effective cytotoxie drugs for individual child with malignant tumor by the chemosensitivity test and discuss the optimal condition of the test. Method Five children with malignant tumors were tested for chemosensitivity by MTT assay. Result The optimal conditions for chemosensitivity were: cell concentration, 5 X 104~5X105, incubation period, 48-72 hours; MTT Concentration, 2.0~2.5g/L. Different cytotoxie drugs demonstrated different effect on the same tumor; The effect of one drug varied considerably among different individuals with the same type of tumor. Conclusion MTT assay, with its simple and rapid features, can be used as a reliable method clinically for antieancer drug sensitivity test in childhood solid tumor.

关 键 词：肿瘤 药物疗法 四氮唑盐 药敏试验 儿童 

分 类 号：R730.53[医药卫生—肿瘤]