不同死亡方式大鼠心肌组织中ATP、ADP和AMP含量比较  

Comparison of the Contents of ATP,ADP,and AMP in the Cardiac Muscle of Rat Killed by Different Manners

作　　者：宋宇[1] 廖林川[1] 颜有仪[1] 杨林[1] 黄红[1] 陈渝[1] 

机构地区：[1]四川大学华西基础医学与法医学院,四川成都610041

出　　处：《法医学杂志》2007年第1期1-3,7,共4页Journal of Forensic Medicine

基　　金：国家自然科学基金资助项目(30371577)

摘　　要：目的比较不同死亡方式对大鼠心肌组织能量物质变化的影响,为判断不同死亡方式及死亡时间提供依据。方法180只大鼠随机分为三组,以不同方式(失血、窒息、断颈)处死,高效液相色谱法检测死后0、0.5、1、1.5、2、3、4、6、8、12、18和24h大鼠心肌组织中的ATP、ADP、AMP含量。结果三组不同死亡方式大鼠心肌组织的ATP、ADP、AMP的含量在大部分时间点做组间比较有明显差异(P<0.05);每组不同死亡方式的大鼠心肌组织中ATP、AMP的含量随时间的变化有明显的差异(P<0.01),而各组中的ADP在死亡一定时间后无明显差异。结论不同死亡方式的大鼠心肌组织中能量物质死后变化可以用作对死亡方式及某种方式不同死亡时间做出判断的依据。Objective The content changes of energy substances in the cardiac muscle of rat killed by different manners were investigated to elucidate evidence that can be used to determine the modes of death and postmortem interval. Methods One hundred and eighty rats were randomly allocated into 3 groups and killed by bleeding, suffocating, and neck breaking, respectively. The contents of ATP, ADP, and AMP in the cardiac muscle of rats killed by the different manners at different death intervals （0, 0.5, 1, 1.5, 2, 3, 4, 6, 8, 12, 18, and 24 h） were measured by HPLC. Results There were significant differences observed in the contents of ATP and AMP in the rats＇ cardiac muscle in different groups at most of the intervals （P〈0.05） and at all of the intervals within the same group （P〈 0.01）, but no differences were found in the ADP contents in any of the group at most of the intervals. Conclusion The content changes of energy substances （ATP and AMP） in the cardiac muscle of dead rats may provide a basis for determination of the death manners and postmortem intervals.

关 键 词：死亡方式 死亡时间 三磷酸腺苷 二磷酸腺苷 一磷酸腺苷 高效液相色谱法 

分 类 号：R-05[医药卫生]