猫胃螺杆菌小鼠感染模型在幽门螺杆菌研究中的应用  

APPLICATION OF MOUSE MODEL INFECTED WITH HELICOBACTER FELIS ON THE STUDY OF HELICOBACTER PYLORI

作　　者：王继德[1] 周殿元[1] 谢国建[1] 张万岱[1] 杨海涛[1] 

机构地区：[1]第一军医大学南方医院全军消化内科研究所

出　　处：《中国微生态学杂志》1996年第6期12-14,共3页Chinese Journal of Microecology

摘　　要：本研究应用幽门螺杆菌的近缘菌猫胃螺杆菌感染的小鼠模型，验证了一个三联治疗方案（羟氨苄青霉素＋次枸橼酸铋＋灭滴灵）的抗菌作用，发现其对Ｈｆ的清除率为９０％，与临床治疗Ｈｐ时的效果相似，说明该模型可用于活体筛检抗Ｈｐ药物，同时探讨了Ｈｆ与腺癌诱癌剂ＭＮＮＧ的协同致癌作用，发现经四个月共同作用后，单纯ＭＮＮＧ组与Ｈｆ感染在胃癌前病变发生率及反应细胞增殖的核仁形成区蛋白嗜银颗粒计数（Ａｇ－ＮＯＲ）、增殖细胞核抗原（ＰＣＮＡ）标记指数等指标均无显著差异，揭示螺杆菌属细菌感染的促癌作用可能是缓慢持久的。Specific Pathogen Free(SPF)Balb/c mice infected with Helicobacter felis(Hf)are used in this study to examine the antibacterial effect of a triple regimen which contains AMO,CBS and MTZ,it is found the clearence rate is 90%,which is similar to that used in Hp therapy clinically.The co-effect of Hf and MNNG which is a strong carcinogen is studied also in this animal model,After innoculation of MNNG to 5 infectious mice for 15 weeks,it is found there are no difference among this and other two control groups with Hf or MNNG only in the occurence of dysplasia,Ag-NOR granule counting and PCNA Labeling Index.This suggests that Hf is't a strong carcinogenic factor in the promoting of animal gastric cancer.

关 键 词：胃螺杆菌感染 动物模型 幽门螺杆菌 

分 类 号：R573[医药卫生—消化系统] R378.99[医药卫生—内科学]