Achromobacter xylosoxidans NS12的分离和对硝基苯酚的降解  

Isolation of Achromobacter xylosoxidans NS12 and Degradation of Nitrophenols

作　　者：万年升[1] 顾继东[1] 黄锦辉[2] 高传德[2] 

机构地区：[1]中国科学院南海海洋研究所LED实验室,广州510301 [2]黄河水资源保护科学研究所,郑州450003

出　　处：《环境科学》2007年第2期422-426,共5页Environmental Science

基　　金：国家高技术研究发展计划(863)项目(2002AA601160);国家自然科学基金委员会-水利部黄河水利委员会联合研究基金项目(50239010)

摘　　要：通过富集培养,从红树林底泥中分离出6株硝基苯酚降解菌,其中Achromobacter xylosoxidansNS12在好氧条件下可耐受小于1.8 mmol/L的邻硝基苯酚(ONP)或3.0 mmol/L的对硝基苯酚(PNP),能以PNP和ONP作为唯一碳源、能源和氮源生长并将其完全矿化,但该菌不能利用间硝基苯酚(MNP)作为唯一碳源和氮源生长.研究发现A.xylosoxidansNS12在降解PNP和ONP组成的混合底物时,PNP的存在可抑制ONP的降解,同时ONP的存在也抑制PNP的降解.此外,在利用PNP和ONP的混合底物时,NS12转化PNP的速率显著地高于转化ONP的速率.红树林底泥中固有的细菌对PNP和ONP具有高效降解作用.A nitrophenols-degrading bacterium, strain NS12, was isolated from a mangrove sediment by enrichment culture under aerobic conditions. Based on the analysis of 16S rDNA gene sequence the isolate was identified as Achromobacter xylosoxidans Strain NS12 was able to metabolize both o-nitrophenol （ONP） and p-nitrophenol （PNP） as the sole source of carbon, energy and nitrogen. However, this strain was not able to use 3-nitrophenol （MNP） as the only source of carbon energy and nitrogen for growth. The study demonstrated that when PNP and ONP occurred as a mixed substrate PNP degradation restrained the degradation of ONP and caused the major carbon source shift from ONP to PNP. Moreover, the results showed nitrophenols could be degraded by the indigenous bacteria in mangrove sediment.

关 键 词：对硝基苯酚 邻硝基苯酚 生物降解 ACHROMOBACTER xylosoxidans 

分 类 号：X172[环境科学与工程—环境科学]