电针“夹脊穴”抑制创伤痛诱发的脊髓FOS蛋白表达  

Electroacupuncture at "Huatuo Jiaji Point" Inhibits the Expression of Fos Protein in Rat Spinal Cord Induced by Traumatic Pain

作　　者：孙文颖[1] 李晓文[1] 娄之聪[1] 刘昱[1] 佟振清[1] 欧阳群[2] 

机构地区：[1]第一军医大学生理教研室,广州510515 [2]第一军医大学中医系

出　　处：《针刺研究》1996年第1期60-64,共5页Acupuncture Research

摘　　要：实验用雄性、成年SD大鼠，以截肢造成创伤痛。用ABC法检测Fos蛋白作为C－fos原癌基因表达的指标，观察电针“夹脊穴”对创伤痛情况下C－fos原癌基因表达的影响，以深入探讨C－fos原癌基因在伤害性信息传递过程中的生物学意义和电针“夹脊穴”镇痛的机理。本实验结果表明，截肢性创伤痛传入信息诱发脊髓内多层神经元C－fos原癌基因表达，其表达产物Fos蛋白，于伤后5h，以后角Ⅰ－Ⅱ及Ⅴ－Ⅵ层内最多；并首次报道电针“夹脊穴”能明显抑制上述表达过程，表现为Fos蛋白形成减少。提示，电针“夹脊穴”缓解术后痛的作用与抑制伤害性传入冲动诱发的脊髓原癌基因表达有关。SD rats were used to study the change of Fos-like immunoreactivity (FLI) in the lumbar spinal cord by traumatic pain following hind limb amputation and the effect of electro-acupuncture (EA) at 'Huatuo Jiaji point' on it. The results showed (1) FLI cells induced by amputation were found in all of the Ⅰ - Ⅱ laminae of the ipsilateral spinal cord, but most of the labeled cells were located in the laminae Ⅰ - Ⅱ and Ⅴ - Ⅵ; (2) EA at IIHuatuo Jiaji point' suppressed the Fos expression follovling amputation, which was indicated by decrease of the number of FLI cells in the spinal cord.It suggests that, the traumatic pain impulse following amputation could activate pain sensitive neurons (PSN) in spinal cord. The impulse of electro-stimulation could suppress activities of these PSN, producing the effect of analgesia

关 键 词：截肢 创伤痛 脊髓 夹脊穴 电针疗法 

分 类 号：R245.97[医药卫生—针灸推拿学] R269.875[医药卫生—中医临床基础]