限食对非酶糖化作用的影响  

THE EFFECT OF DIETARY RETRICTION ON NONENZYMATIC GLYCATION

作　　者：程胜求[1] 赵法伋[1] 郭俊生[1] 钮燕[1] 陈洪章[1] 

机构地区：[1]第二军医大学军队卫生教研室,上海海军医学研究所新药研究中心

出　　处：《营养学报》1996年第2期139-144,共6页Acta Nutrimenta Sinica

摘　　要：本研究通过限制小鼠的摄食量（６０％）而维生素和矿物质与对照小鼠等量供给，建立了限食模型。通过制备ＡＧＥ（ＡｄｖａｎｃｅｄＧｌｙｃａｔｉｏｎＥｎｄｐｒｏｄｕｃ－ｔｓ，高级糖化终产物）特异性抗血清，采用竞争性ＥＬＩＳＡ（ＥｎｚｙｍｅＬｉｎｋｅｄＩｍ－ｍｕｎｏｓｏｒｂｅｎｔＡｓｓａｙ，酶联免疫吸附试验）测定了小鼠组织中ＡＧＥ的含量，发现限食可广泛减少体组织中ＡＧＥ的累积。放射受体分析法测定８月龄小鼠限食１２个月盾腹腔巨噬细胞的ＡＧＥ受体，发现其数量比对照鼠高１０倍左右，每个细胞有１．０２９×１０６；其结合常数比对照鼠高出１倍，达１．４２×１０８ｍｏｌ－１。结合前人的研究结果，提出了限食延寿机制的非酶糖化假说。o establisli a dietary restriction(DR)model,fced of DR mice was restricted to 60% of control with the same intakes of vitamins and minerals. Anti-AGE antiserum was prepared and used in a newly established compe-titive ELISA to determine the AGE content of some tissues.The results indicated that AGE accumulated less in tlie tissues after dietary restriction. In radiolabeled ligand binding experiments,it was found that after 12 mon-ths of DR,peritoneal macrophages of 20 month old mice displays 1.029 ×106 AGE receptors per cell,10 times over control,and affinity constant Ka is 1.42×108mol-1,twice of control.Based on the results in this investigation and those reported in literature,the nonenzymatic glycation hypothesis was proposed for the mechanism of DR action.

关 键 词：限食 高级糖化终产物 衰老 

分 类 号：R154[医药卫生—营养与食品卫生学] R339.38[医药卫生—公共卫生与预防医学]