耐受型抗原递呈细胞对小鼠角膜植片免疫排斥的影响  

TGF-β_2 and corneal antigen co-cultured antigen presenting cell and graft rejection in a mouse model

作　　者：赵萌[1] 邹留河[1] 

机构地区：[1]首都医科大学附属北京同仁医院眼科中心,北京100730

出　　处：《眼科研究》2007年第2期94-97,共4页Chinese Ophthalmic Research

摘　　要：目的建立小鼠同种异体角膜移植模型,观察静脉注入抗原递呈细胞对术后免疫排斥的影响。方法建立BALB/c→C57BL/6小鼠角膜移植模型。随机分2组,静脉注入耐受型或普通抗原递呈细胞。根据植片混浊评分,判断植片排斥情况。随机取两组小鼠脾脏检测CD4+NKT百分比和IL-10质量分数。结果与对照组相比,实验组移植排斥出现晚(19d±1.6d,P=0.009)。术后脾脏CD4+NKT百分比、IL-10质量分数相对恒定。结论小鼠角膜移植术后耐受型抗原递呈细胞静脉注射可延长植片存活时间。Objective A strategy to generate tolerant antigen presenting cells （APCs） using intervenous injection of adherent peritoneal exudate cells （PEC） co-cultured with TGF-β2 and antigen has been proven to be effective in suppression of DTH generated on pre-scnsitized site. We set our purpose on determination of the effect of intervenous injection of tolerant APCs on the mouse corneal allograft rejection. Methods Ninety-six C57BL/6 mice received cornea from BALB/c mice donors. After keratoplasty,the mice randomly received intravenous injection of TGF-β2 and particle BALB/c corneal antigen co-cultured APCs （experimental group,12 mice） or only later （control group, 12 mice） or free-serum cell culture media（ blank control group, 12 mice）. The percentage of CD4＋ nature killer T cell（ NKT cell） in spleen and interleukin 10 （IL-10） in the spleen surpernant fluid were measured from 5 mice each group per week for 1 month. Other mice were randomly divided into 3 groups for clinical scoring by slit-lamp per day to determine the rejection occurrence for 1 month. Results The median survival time of corneal graft in experimental group was 19±1.6 days ; while that in control group and blank control group was 9±0.7 days （ P = 0. 008 9 ） and 7±0. 7days（ P = 0. 000 3 ）respectively, showing a significant difference. CD4 ＋ NKT cell percentage was increased in spleen and IL-10 concentration and kept a stable level at 1,7, 14,21,30 days in experimental group but exhibited a fluctuation during the early postoperative days in control group. Conclusion The intravenous injection of TGF-β2 and corneal antigen co-cultured APCs is effective in normal-risk recipients during the early postkeratoplasty period. The stable increase of CD4 ＋ NKT cell percentage in spleen and steady concentration of IL-10 may contribute to the formation of anterior chamber-associated immune deviation.

关 键 词：角膜移植 移植物排斥 自然杀伤性T细胞 前房相关性免疫偏离 

分 类 号：R779.65[医药卫生—眼科]