c-myc反义核酸对乳腺癌MCF-7细胞生长及hTERT基因表达的影响  

Effect of c-myc Antisense Oligodeoxynucleotides on Cytology and Human Telomerase Reverse Transcriptase Gene in MCF-7 Cells

作　　者：马莉[1] 税青林[1] 张莉娟[1] 彭春 赵小平[1] 

机构地区：[1]四川省泸州医学院医学生物学与遗传学教研室,泸州646000

出　　处：《肿瘤防治研究》2007年第1期14-17,共4页Cancer Research on Prevention and Treatment

基　　金：四川省教育厅自然基金重点资助项目(2002A068)

摘　　要：目的探讨脂质体介导c-myc基因反义寡核苷酸(Antisense phosphorothioate oligodeoxynucle-otide,ASODN)对MCF-7细胞生长及其人体端粒酶逆转录酶(humantelomerasereversetranscriptase,hTERT)基因表达的影响。方法将c-myc正、反义寡核苷酸(ODN)分别导入各组MCF-7细胞中,MTT法、逆转录-聚合酶链法及流式细胞术分别检测各组细胞生长情况、hTERTmRNA的表达水平以及细胞凋亡率。结果c-mycASODN转染MCF-7细胞24h后,细胞生长受到抑制(P<0.05),并且hTERTmRNA表达明显降低;随着反义核酸作用时间的延长,凋亡细胞数目逐渐增多,细胞生长及hTERT表达的下降随时间延长逐渐明显。对照组、LR-SODN组、LR-ASODN24h组与LR-ASODN48h、72h组相比有明显统计学差异(P<0.05)。结论c-myc反义寡核苷酸能显著下调细胞中hTERT基因的表达活性,诱导MCF-7细胞凋亡,并抑制MCF-7细胞生长;在一定程度上,其效果与时间呈正相关。Objective To investigate the effects of c-myc ASODN on MCF-7 cells growth and the expression of hTERT gene in MCF-7 cells. Methods After transfecting c-myc SODN and ASODN to MCF-7 cells, we measured the proliferation, apoptosis rates and the expression of hTERT mRNA respectively by MTT method, flow cytometry analysis and RT-PCR. Results When c-myc ASODN affected MCR-7 cells at 24h, cells growth was inhibited significantly （P〈0. 05） with hTERT gene expression of MCF-7 cells descending significantly （P〈0. 01）. The apoptosis rates of the ASODN-treated cells rised significantly at 48h. The described effects of suppression on cells rised with time increasing. Conclusion c-myc ASODN can effectively inhibit the MCF-7 cells growth, induce apoptosis, and down-regulate hTERT gene expression in MCF-7 cell. The effects correlated with time to a degree.

关 键 词：乳腺肿瘤 c-myc 人端粒酶逆转录酶 反义核酸 MCF-7细胞 

分 类 号：R73-362[医药卫生—肿瘤] R737.9[医药卫生—临床医学]