微小RNA抑制胚胎细胞MAD2基因的表达  

MicroRNA depresse the expression of MAD2 gene in trophoblastic cells

作　　者：施琼[1] 翁亚光[2] 徐远久[2] 蔡燕[2] 蒋洪彦[2] 

机构地区：[1]重庆医科大学基础医学院放射医学教研室 [2]重庆医科大学临床检验诊断学实验室,重庆400016

出　　处：《重庆医科大学学报》2007年第2期138-141,共4页Journal of Chongqing Medical University

基　　金：重庆医科大学校办课题(NSFLX200519)

摘　　要：目的:探讨微小RNA(microRNA)抑制胚胎细胞MAD2基因表达的机理。方法:用定量real-time PCR比较microRNA转染前后的胚胎细胞内源性基因MAD2 mRNA表达水平,同时用western blot比较转染前后Mad2蛋白的表达水平。结果:对照组的MAD2基因mRNA拷贝数/GAPAH mRNA拷贝数为0.1780±0.0688,而转染microRNA重组质粒后,实验组的mRNA比值分别为0.1778±0.0689和0.1778±0.0670,经统计分析,两实验组细胞的内源性MAD2基因mRNA水平与对照组无显著性差异;而其中一实验组的蛋白水平在转染后有明显降低。结论:microRNA主要在翻译水平调节哺乳动物细胞内基因的表达,为研究基因表达的调控机制奠定了良好的基础。Objective：To discuss the mechanism of down-regulated expression of MAD2 gene in trephoblastic cells by microRNA. Methods：Endogenous MAD2 mRNA level was compared by using quantitative real-time PCR before and after transfected with mieroRNA plasmid;and the Mad2 protein level was compared by western blot.Results：Ratio of MAD2 mRNMGAPDH mRNA of control group was 0.1780 ±0.0688,and after transfected with microRNA recombinant plasmid was 0.1778 ± 0.0689 and 0.1778 ± 0.0670respectively.Statistical study of samples showed they have no instinct different;otherwise the protein level in one of the experimental groups was siguificantly decreased after transfected with microRNA plamid.Conclusion：This study established mieroRNA can down-regulate gene expression in mammalian cells at translation level,it is a good foundation to study on the control of gene expression.

关 键 词：微小RNA MAD2基因 定量RT-PCR 

分 类 号：R394.2[医药卫生—医学遗传学] Q343.2[医药卫生—基础医学]