Nimesulide对乳腺癌细胞株MMP-2、MMP-9基因表达的影响  

The effect of nimesulide on the expressions of MMP-2、MMP-9mRNA in breast cancer cell lines in vitro

作　　者：郭贵龙[1] 李卓英[2] 周素梅[1] 林包荣[1] 张筱骅[1] 

机构地区：[1]温州医学院第一附属医院肿瘤外科,浙江温州325000 [2]温州医学院第一附属医院ICU,浙江温州325000

出　　处：《温州医学院学报》2007年第1期52-55,共4页Journal of Wenzhou Medical College

基　　金：温州医学院科研发展基金资助项目(2003×01)。

摘　　要：目的:探讨环氧化酶-2选择性抑制剂nimesulide对乳腺癌细胞株MMP-2、MMP-9mRNA表达的影响。方法:应用逆转录聚合酶链反应(RT-PCR)的方法,检测不同浓度nimesulide作用体外培养的MDA-MB-231、MCF-7人乳腺癌细胞株48h,MMP-2、MMP-9mRNA的变化。结果:50 μ mol/L nimesulide明显抑制MDA-MB-231细胞MMP-2mRNA的表达,100 μ mol/L时其几乎完全抑制;100 μ mol/L nimesulide显著抑制MCF-7细胞MMP-2mRNA的表达,200 μ mol/L时完全抑制其基因的表达。50 μ mol/L nimesulide明显抑制MDA-MB-231、MCF-7细胞MMP-9mRNA的表达,随着浓度的增加,MMP-9mRNA逐渐被抑制。结论:nimesulide以剂量依赖性方式下调MDA-MB-231、MCF-7乳腺癌细胞株MMP-2、MMP-9mRNA的表达,而MDA-MB-231细胞对nimesulide更敏感,这种作用是COX-2非依赖性途径,为nimesulide用于转移乳腺癌的治疗与预防提供了实验依据。To investigate the effect of nimesulide of the expressions of MMP-2, MMP-9 in human breast cancer cell lines, including MDA-MB=231 （overexpression COX-2） and MCF-7 （no expression of COX-2）. Method RT-PCR was used to determine the levels of expressions of MM=2, MMP-9 mRNA in HDA-MB-231. MCF=7 cells treated at different concentrations of nimesulide for 48h, respectively. Results： Nimesulide significantly suppressed MMP-2, MMP-9 mRNA expressions in MDA-MB-231 cell line at 50μmol/L nimesulide, and in MCF-7 cell line at 100 - 200 μ mol/L, in a does-dependent manner. Conclusions： Nimesttlide could down-regulate the amount of MMP-2, MMP-9 mRNA expressions in a does=dependent manner. The expressions of these genes were more sensitive to nimesulide in MDA-MB-231 than that in MCF-7 cell line. The mechanism, which wasn＇t dependent on COX-2 expression, may provide the experimental evidence for nimesulide used for the prevention and treatment of the metastatic breast cancer.

关 键 词：乳腺癌 细胞株 选择性环氧化酶-2抑制剂 MMP-2 MMP-9 基因 

分 类 号：R737.9[医药卫生—肿瘤]