生防菌ZJY-1抑菌作用的初步研究  

Antagonistic activity of biocontrol bacterium ZJY-1 Brevibacillus brevis

作　　者：张昕[1] 张立钦[1] 马良进[1] 林海萍[1] 毛胜凤[1] 张炳欣[2] 

机构地区：[1]浙江林学院林业与生物技术学院,浙江临安311300 [2]浙江大学农业与生物技术学院,浙江杭州310029

出　　处：《浙江林学院学报》2007年第1期91-95,共5页Journal of Zhejiang Forestry College

基　　金：国家自然科学基金重点资助项目(30230250)

摘　　要：通过测定发酵上清液和细胞破碎液对供试黄瓜枯萎病菌Fusarium oxysporiumf. cucumerinum菌丝生长和分生孢子萌发的抑制作用,初步研究了植物病原生防细菌ZJY-1 Brevibacillus brevis的拮抗活性。结果表明,该菌株主要通过分泌到胞外的次生代谢产物抑制病原菌的生长。与对照和细胞破碎液处理相比,病原菌菌丝在含有发酵上清液的培养基上生长明显受到抑制;由发酵上清液制备的拮抗粗提物显著抑制病原孢子的萌发和芽管的伸长,抑制率分别为96.0%和82.0%。Preliminary study on antagonistic activity of biocontrol bacterium BrevibaciUus brevis ZJY-1 was made by analyzing the inhibitory effect of cellular extraction and cell-free supernatant to mycelium growth and conidial spore germination of Fusarium oxysporium f. cucumerinum. The results showed that the growth of Fusarium oxysporium f. cucumerinum was inhibited mainly by metabolites excreted outside by the strain. In medium contained with cellfree supernatant, Mycelium growth of Fusarium oxysporum f. cucumerinum was obviously affected compared with that cultured in check and cellular extraction-contained medium. The metabolites derived from supernatant could evidently inhibit germination and tube growth of conidia, the inhibit rate was up to 96.0% and 82.0% respectively.

关 键 词：微生物学 生防细菌 生物防治 抑菌作用 植物病害 次生代谢产物 

分 类 号：S476[农业科学—农业昆虫与害虫防治] Q935[农业科学—植物保护]