5种海水鱼同工酶表达的组织特性及其电泳的初步分析  

THE SPECIFIC EXPRESSION OF ISOZYMES IN DIFFERENT TISSUES AND PRELIMINARY BIOCHEMICAL GENETIC ANALYSIS BASED ON THE ELECTROPHORESIS OF FIVE MARINE FISHES

作　　者：王可玲[1] 尤锋[1] 徐成[1] 吴谡琦[1] 

机构地区：[1]中国科学院海洋研究所,青岛266071

出　　处：《海洋与湖沼》1996年第6期626-631,共6页Oceanologia Et Limnologia Sinica

基　　金：国家科委攀登计划B项目!PDB6-5-2

摘　　要：于1994年7—12月，在山东、福建沿海采集真鲷、黑鲷、牙鲆、鲈和小黄鱼等5种鱼63尾692个生化样品，应用水平淀粉胶和垂直聚丙烯酰胺凝胶电泳方法，对5种鱼的肌肉、眼睛、肝脏、心脏、鳍、鳃、肾脏共7种组织的23种同工酶进行初步分析。结果为：1．选出酶活性强，基因座位表达稳定、清晰的肌肉、眼睛、肝脏、心脏4种组织，用于以上5种鱼的常规性生化遗传分析；2．筛选出适于电泳分析的缓冲液系统有TCl，EBT，TG等3种以及14种图谱清晰、分辨率高的同工酶（LDH，MDH，IDHP，MEP，ADH，SDH，G6PDH，CAT，GDH，PGM，PGDH，GP，EST，Pm，SOD）和肌浆蛋白（Pm）；3．对其中的LDH，IDHP，ADH，GDH和BST5种同工酶进行生化遗传分析，包括每种酶的亚基结构、编码座位以及多态座位等位基因数等。Sixty-three fishes belonging to 5 different species (Pagrusomus major, Sparus macrocephalus, Paralichthys olivaceus, Lateolabrax Japonicus, Pseudosciaena polyactis) were collected from the coastal waters of Shandong and Fujian provinces from July to December in 1994. Biochemical samples were extracted by homogenizing from seven tissues (muscle,eye, liver, heart, gill, fin and kidney) of each species separately. Horizontal starch gel and ventical polyacrylamide gel electrophoresis were used in examining 692 samples. Four kinds of buffer systems, TC1, TC2 and EBT for starch gel and TG for polyacrylamide gel electrophoresis, were tested. Altogether 22 isozymes and a prowin were examined through different kinds of hssue or different kinds of buffer systems. only EST, Pm, and SOD were electrophoresed by polyaCrydride ge, the others by hawh gel The results showed the isozymes expedon was twhiy hssue specific. Among the buffer systems tested, TC1, EBT and TG, seemed very useful for the future applicahon due to thdr good resoinhon of isozymes and a protrin, Which eAnbital stable expression and high level of adivity in four kinds of hssue whthe, eye, liver and hcart) during the forphotals (Tab. 1).Furthennre, two buffer systems supporting the stareh ge electrophoresis gave good resolu tion of 12 isozymes: TC1 to LDH, MDH, IDHP and MEP; EBT to ADH, SDH,G6PDH, CAT, GDH, PGM, PGDH, and GPI. Another buffer sydri, TG Was a superior to EST, Pm and SOD for polyacrylamide gel electrophoresis (Tab.2, Tab.3). Finally, based on the zymograms, five isozymes, e.g. LDH, IDHP, ADH, GDH and EST (Plate I), were analynd genhcally on thdr subunit structure, coding gene led and nUmbers as Well as thdr specific expression in there five marine fishes.

关 键 词：同工酶 电泳 组织特性 海水鱼 真鲷 牙鲆 

分 类 号：Q959.483[生物学—动物学] Q959.486