^（125）I－抗AFP制备的研究  

A STUDY ON THE PREPARATION OF 125 I Ab AFP

作　　者：周德南[1] 甘友全[1] 吴英德[1] 宋向群[1] 杨克政[1] 黄秉琰[1] 

机构地区：[1]广西肿瘤防治研究所

出　　处：《广西医科大学学报》1996年第4期7-9,共3页Journal of Guangxi Medical University

基　　金：广西区科委资助

摘　　要：用亲和层析法提纯马抗ＡＦＰ，采用氯胺Ｔ改良法标记１２５Ｉ，经ＳｅｐｈａｄｅｘＧ５０柱分离，以ｒ计数与紫外同步监测进行标记物的收集。并对１２５Ｉ－抗ＡＦＰ制备方法及其稳定性等进行探讨和测定。结果：１２５Ｉ和抗ＡＦＰ的利用率分别为６０％～８０％和５０％～７３％；标记率为６５％～８３％，１２５Ｉ－抗ＡＦＰ的比放射性为５５．５～７４ＭＢｑ／ｍｇ；放化纯度５ｄ内为９７％～９８％，１４ｄ为９３％；抗体活性标记后与标记前基本不变，４℃保存５ｄ和１４ｄ均无降低。表明１２５Ｉ－抗ＡＦＰ的生物活性及稳定性良好。AFP antibody (Ab AFP) from horse serum was purified by affinity chromatography, 125 I was marked by improved P toluene sulphonamide method,and the marker was collected by r counter and ultrariolet way after it was separated by sephadex G 50 The method of preparation and the stablity of 125 I Ab AFP were examined Result:Utilixation rate of 125 I and Ab AFP was 60%￣80% and 50%￣73% respectively Marker rate:65%￣83%; 125 I Ab AFP's specific radioactivity:55 5￣74MBq/mg;radiochemical purity:97%￣98%(five days) and 93%(fourteen days) The activity of the antibody after marking was very similar to that before marking,and did not decrease after preservation for five days or fourteen days at 4℃ The result shows that the biologic activity and stablity of 125 I Ab AFP are good

关 键 词：亲和层析 碘125 抗AFP 放射免疫法 肝肿瘤 

分 类 号：R735.705[医药卫生—肿瘤] R392－33[医药卫生—临床医学]