白藜芦醇对胃癌细胞SGC7901增殖与凋亡的影响  

Effect of resveratrol on the proliferation and apoptosis of gastric cancer cell line SGC7901

作　　者：王学清[1] 王贺玲[2] 李岩[1] 田丰[1] 王颖[1] 

机构地区：[1]中国医科大学附属盛京医院消化内科,辽宁省沈阳市110004 [2]沈阳市第一人民医院消化内科,辽宁省沈阳市110044

出　　处：《世界华人消化杂志》2007年第4期340-345,共6页World Chinese Journal of Digestology

摘　　要：目的:探讨白藜芦醇(Res)对胃癌细胞SGC7901增殖与凋亡的影响．方法:MTT法检测不同浓度(0,10,20,50,100．200μmol/L)Res处理24,48,72 h对SGC7901细胞的抑制率．流式细胞仪采用AnnexinV和PI双染检测细胞的早期凋亡率．电镜扫描观察SGC7901细胞形态学改变,分光光度法检测caspase-3活性．结果:Res在20-300μmol/L浓度范围内．以浓度和剂量依赖的方式抑制胃癌细胞的增殖(P＜0．01)．经0,10,20,50,100,200μmol/L Res处理24 h后,细胞凋亡率分别为1．17%,3．73%, 8．75%,23．35%,63．97%和70．10%,晚期凋亡和坏死的细胞比例分别为5．66%,7．22%,9．86%, 6．91%,12．51%及11．98%,各组之间差异不大．电镜下见到典型的细胞凋亡的形态学改变．100μmol/L Res处理后6 h caspase-3活性增加,18 h达高峰,24 h后下降,48 h仍高于正常(0．135±0．036 vs 0．069±0．008,P＜0．05)．经20,50,100μmol/L Res处理18 h后的细胞的caspase-3活性分别为0．169±0．017．0．247±0．028,0．353±0．044(P＜0．01),较对照组(0．063±0．006)分别增加2．68倍、3．92倍和5．61倍．结论:Res通过诱导caspase-3活性增加以时间依赖和浓度依赖的方式抑制SGC7901细胞增殖,诱导细胞凋亡．AIM： To investigate the effect of resveratrol on the proliferation and apoptosis of gastric cancer cell line SGC7901. METHODS： Gastric cancer cell line SGC7901 was cultured and treated with different concentrations （0, 10, 20, 50, 100, 200 μmol/L） of resveratrol for 24, 48 and 72 hours. The growth inhibition rate of SGC7901 cells was detected by MTT method, and cell apoptosis was analyzed by flow cytometry （FCM） using annexin V/propidium iodide （PI） double staining. The morphological changes of SGC7901 cells were observed by electron microscopy, and caspase-3 activity was assessed by colorimetric assay. RESULTS： Resveratrol inhibited the proliferation of SGC7901 cells in a time- and concentration-dependent manner ranging from 20 to 300 μmol/L （P 〈 0.01）. After resveratrol treatment at 0, 10, 20, 50, 100, and 200 μmol/L for 24 hours, the apoptosis rate of SGC7901 cells was 1.17%, 3.73%, 8.75%, 23.35%, 63.97% and 70.10%, while the necrosis rate was 5.66%, 7.22%, 9.86%, 6.91%, 12.51% and 11.98%, respectively. Electron microscopy showed typical morphological changes in SGC7901 cells. After 100 μmol/L res- veratrol treatment for 6 hours, the activity of caspase-3 was increased, and reached the peak at the 18^th hour, then declined gradually. Caspase-3 activity was still higher at the 48^th hour than the normal level （0.135 ± 0.036 vs 0.069 ±0.008, P 〈 0.05）. After SGC7901 cells were treated with 20, 50, and 100 μmol/L resveratrol for 18 hours, the activity of caspase-3 was 2.68, 3.92 and 5.61 folds as high as that in control group （0.169 ± 0.017, 0.247 ±0.028, 0.353 ±0.044 vs 0.063 ± 0.006, P 〈 0.01）. CONCLUSION： Resveratrol can inhibit the proliferation and induce apoptosis of SGC7901 cells in concentration- and time- dependent manners by increasing caspase-3 activity.

关 键 词：白藜芦醇 胃癌 增值 凋亡 CASPASE-3 

分 类 号：R735.2[医药卫生—肿瘤]