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作 者:王学清[1] 王贺玲[2] 李岩[1] 田丰[1] 王颖[1]
机构地区:[1]中国医科大学附属盛京医院消化内科,辽宁省沈阳市110004 [2]沈阳市第一人民医院消化内科,辽宁省沈阳市110044
出 处:《世界华人消化杂志》2007年第4期340-345,共6页World Chinese Journal of Digestology
摘 要:目的:探讨白藜芦醇(Res)对胃癌细胞SGC7901增殖与凋亡的影响.方法:MTT法检测不同浓度(0,10,20,50,100.200μmol/L)Res处理24,48,72 h对SGC7901细胞的抑制率.流式细胞仪采用AnnexinV和PI双染检测细胞的早期凋亡率.电镜扫描观察SGC7901细胞形态学改变,分光光度法检测caspase-3活性.结果:Res在20-300μmol/L浓度范围内.以浓度和剂量依赖的方式抑制胃癌细胞的增殖(P<0.01).经0,10,20,50,100,200μmol/L Res处理24 h后,细胞凋亡率分别为1.17%,3.73%, 8.75%,23.35%,63.97%和70.10%,晚期凋亡和坏死的细胞比例分别为5.66%,7.22%,9.86%, 6.91%,12.51%及11.98%,各组之间差异不大.电镜下见到典型的细胞凋亡的形态学改变.100μmol/L Res处理后6 h caspase-3活性增加,18 h达高峰,24 h后下降,48 h仍高于正常(0.135±0.036 vs 0.069±0.008,P<0.05).经20,50,100μmol/L Res处理18 h后的细胞的caspase-3活性分别为0.169±0.017.0.247±0.028,0.353±0.044(P<0.01),较对照组(0.063±0.006)分别增加2.68倍、3.92倍和5.61倍.结论:Res通过诱导caspase-3活性增加以时间依赖和浓度依赖的方式抑制SGC7901细胞增殖,诱导细胞凋亡.AIM: To investigate the effect of resveratrol on the proliferation and apoptosis of gastric cancer cell line SGC7901. METHODS: Gastric cancer cell line SGC7901 was cultured and treated with different concentrations (0, 10, 20, 50, 100, 200 μmol/L) of resveratrol for 24, 48 and 72 hours. The growth inhibition rate of SGC7901 cells was detected by MTT method, and cell apoptosis was analyzed by flow cytometry (FCM) using annexin V/propidium iodide (PI) double staining. The morphological changes of SGC7901 cells were observed by electron microscopy, and caspase-3 activity was assessed by colorimetric assay. RESULTS: Resveratrol inhibited the proliferation of SGC7901 cells in a time- and concentration-dependent manner ranging from 20 to 300 μmol/L (P 〈 0.01). After resveratrol treatment at 0, 10, 20, 50, 100, and 200 μmol/L for 24 hours, the apoptosis rate of SGC7901 cells was 1.17%, 3.73%, 8.75%, 23.35%, 63.97% and 70.10%, while the necrosis rate was 5.66%, 7.22%, 9.86%, 6.91%, 12.51% and 11.98%, respectively. Electron microscopy showed typical morphological changes in SGC7901 cells. After 100 μmol/L res- veratrol treatment for 6 hours, the activity of caspase-3 was increased, and reached the peak at the 18^th hour, then declined gradually. Caspase-3 activity was still higher at the 48^th hour than the normal level (0.135 ± 0.036 vs 0.069 ±0.008, P 〈 0.05). After SGC7901 cells were treated with 20, 50, and 100 μmol/L resveratrol for 18 hours, the activity of caspase-3 was 2.68, 3.92 and 5.61 folds as high as that in control group (0.169 ± 0.017, 0.247 ±0.028, 0.353 ±0.044 vs 0.063 ± 0.006, P 〈 0.01). CONCLUSION: Resveratrol can inhibit the proliferation and induce apoptosis of SGC7901 cells in concentration- and time- dependent manners by increasing caspase-3 activity.
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