人GITR基因的克隆和序列分析  

Molecular cloning and sequence analysis of human Glucocorticoid-inducible tumor necrosis factor receptor gene

作　　者：仝佳[1] 王胜军[1] 毛朝明[1] 杨云良[2] 陈君[1] 杨敏[1] 

机构地区：[1]江苏大学医学技术学院免疫学系,江苏镇江212001 [2]江苏大学附属医院眼科,江苏镇江212001

出　　处：《江苏大学学报（医学版）》2007年第1期15-18,共4页Journal of Jiangsu University：Medicine Edition

基　　金：国家自然科学基金资助项目(30300169);江苏省社会发展基金资助项目(BS2000026);江苏大学青年自然科学基金资助项目(2241270005)

摘　　要：目的:克隆人GITR基因全长编码区的cDNA,同时对其序列进行分析。方法:采用RT-PCR方法,从正常人外周血单个核细胞获得GITR基因的cDNA,克隆至pGEM-T载体,选择阳性克隆并进行序列测定。结果:扩增得到的人GITR基因编码区cDNA的全长726 bp,编码241个氨基酸残基,与GeneBank注册的序列完全一致。结论:获得人类GITR基因的克隆,为进一步研究其生物学功能奠定了基础。Objective： To clone and analyze a full-length eDNA encoding human glucocorticoid-inducible tumor necrosis factor receptor（GITR） gene. Methods： The eDNA of GITR was amplified by RT-PCR using the total RNA extracted from normal human peripheral blood mononuclear cell（PBMC）. The PCR product was inserted into pGEM-T vector and then transformed into E. coli DH5α. The positive recombinant clone was analyzed by digestion of restriction endonuclease and DNA sequencing. Results： The eDNA of human GITR had a complete open reading frame with a length of 726 bp,which encoded a product of 241 amino acid, and shared 100% homology with the sequence of mRNA for GITR in Genbank. Conclusion： The eDNA of hGITR was cloned successfully, which posed a basis for further researching on its biological function.

关 键 词：人类糖皮质激素诱导的肿瘤坏死因子受体 CDNA克隆 RT-PCR 调节性T细胞 

分 类 号：R394[医药卫生—医学遗传学]