白消安诱导构建胎鼠肢体畸形模型的研究  

Establishment of a mouse model of limb malformation induced by Busulfan

作　　者：宋燕妮[1] 关德宏[2] 庞建华[2] 王冰[2] 

机构地区：[1]哈尔滨医科大学附属肿瘤医院头颈外科,黑龙江哈尔滨150081 [2]哈尔滨医科大学附属第二医院整形外科

出　　处：《中国美容整形外科杂志》2007年第1期71-75,共5页Chinese Journal of Aesthetic and Plastic Surgery

摘　　要：目的用易获得的化学药物建立大鼠四肢畸形发生率稳定、畸形类型特异的动物模型。方法采用抗肿瘤类致畸药物白消安作为受试物,观察不同剂量和不同给药时间的胎仔畸形率、畸形类型及特征。结果在大鼠受孕第12天(GD12),一次经口给予白消安25mg/kg时,胎仔畸形类型主要为肢体畸形。肢体畸形率以活胎计为37.9%(33/87),以窝计为61.5%(8/13)。畸形类型常见于多指(趾)和缺指(趾),掌跖骨缺失和骨化不全发生率也较高。此外,还发生胫骨缺失和骨化不全,在观察大体形态时所见的短肢是由胫腓骨缺失和发育不全所致。四肢畸形的发生率和严重程度存在着不对称性,后肢较前肢出现率高,缺指(趾)畸形较其他畸形出现率高。结论成功建立大鼠肢体畸形动物模型,为进一步分析研究肢体发育畸形的分子机制和潜在原因奠定了基础。Objective To develop a convenient and stable animal model of limbs malformation using a chemiead teratogens. Methods Busulfan is a mutagenic teratogen that was used to induce SD rat embryo limb malformation. The type and character of malformation, together with the rate and of limb deject inducdad by the Busulfan at different dosages and gestational exposure times were observed. Results Limb deject was the main type of defect occurring on gestational day 12 with 25 mg/kg of Busulfan. Occurrence and severity of these were unsymmetric, with malformed limbs on the lejt side were more frequently than limbs on the right side, and malformed hind limbs were more frequently than forelimbs. The incidence of fetal limb dejects was 37.9 % in total living offspring and 61.5 96 for litters overall. The phenotypes of affected limb mainly of oligodactyly, truncated limb and polydactyly. Skeletal preparation showed that the loss of tibia and fibula led to the truncated limbs. Conclusion An animal model of limb malformation for studying the molecular mechanism of chemical teratogev.s has been established successfully.

关 键 词：白消安 动物模型 肢体畸形 发育 

分 类 号：R-332[医药卫生]