梅毒RPR、TPPA、TPHA试剂盒临床血清实验比较  

Compare effect of three kits in detecting syphilis antibody

作　　者：王彦[1] 石宏宴[2] 刘志敏[1] 赵赫男[3] 崔奕文[4] 

机构地区：[1]大连医科大学第二临床学院实验中心,辽宁大连116027 [2]大连医科大学第二临床学院检验科,辽宁大连116027 [3]大连医科大学病理生理学教研室,辽宁大连116027 [4]大连市中心医院检验科,辽宁大连116033

出　　处：《大连医科大学学报》2007年第1期76-77,共2页Journal of Dalian Medical University

摘　　要：[目的]了解快速血浆反应素环状卡片试验(rapid plasma regain circle card,RPR)、螺旋体血球凝结试验(treponema pallidum gemagglutination assay,TPHA)、螺旋体明胶凝结试验(treponema pallidum particle agglutina-tion,TPPA)在诊断梅毒中的意义。[方法]采用三种试剂盒,检测60例经临床确诊的梅毒患者、44例健康体检者血清中的梅毒抗体。[结果]在60例梅毒患者血清中,RPR试验50例(20例Ⅰ期梅毒和30例Ⅱ期梅毒)呈阳性反应,阳性检出率为83.3%;TPHA试验38例(8例Ⅰ期梅毒和30例Ⅱ期梅毒)呈阳性反应,阳性检出率为63.3%;TPPA试验60例(20例Ⅰ、30例Ⅱ期和10例早期潜伏梅毒)均呈阳性反应,阳性检出率为100%。在44例健康者血清中,三种试剂均未检测到梅毒抗体。[结论]TPPA试剂具有高度敏感性及特异性,不仅能准确鉴定Ⅰ、Ⅱ期梅毒,还可较早地筛选出早期潜伏梅毒,缩短了梅毒的窗口期。[ Objective ] To understand the significance of RPR （ rapid plasma regain circle card）, TPHA （ treponema paUidum gemagglutination assay ）and TPPA（treponema pallidum particle agglutination）in diagnosing syphilis. [ Methods] Using three kits to detect the syphilis antibody in blood serum of 60 cases syphilis patients and 40 eases healthy persens. [ Results ] In 60 eases syphilis patients blood serum, 50 eases （ineluding 20 eases first stage and 30 eases seeond stage syphilis ） were positive by RPR, the positive ratio was 83.3% ; 38 eases （ including 8 eases first stage and 30 eases second stage syphilis ） were positive by TPHA, the positive ratio was 63. 3% ; all cases （ineluding 20 eases first stage, 30 eases seeond stage and 10 eases ineubated syphilis ） were positive by TPPA , the positive ratio is 100%. In 44 eases healthy persons blood serum, all eases are negative by three kits. [ Conehsion] TPPA has highly sensibility and specificity in detecting syphilis antibody. It not only can definite the staging of syphilis, but also ean detect the ineuba- ted syphilis early and shorten the syphilis window phase.

关 键 词：梅毒 RPR TPPA TPHA 

分 类 号：R446.6[医药卫生—诊断学]