核仁组成区嗜银蛋白在子宫内膜病变组织中的研究  

Study on AgNOR in Endometrial Diseases

作　　者：马晓欣[1] 金英楠[1] 赵艳辉[1] 苗青[1] 张忠福[1] 李晓晗[2] 

机构地区：[1]中国医科大学附属二院盛京医院妇产科 [2]中国医科大学附属二院盛京医院病理科,沈阳110004

出　　处：《生殖与避孕》2007年第1期34-37,共4页Reproduction and Contraception

基　　金：辽宁省2002年博士启动基金(20021036)

摘　　要：目的：探讨核仁组成区嗜银蛋白(AgNOR)在子宫内膜样腺癌早期诊断与预后中的作用。方法：采用银染法染色,检测子宫内膜增殖症患者(25例)、子宫内膜非典型增生患者(19例)、子宫内膜样腺癌患者(63例)检测病变内膜组织中单位细胞核内AgNOR的颗粒数,同时观察其形态学变化；并以因患宫颈原位癌子宫切除后的正常子宫内膜组织(25例)为对照。结果：AgNOR每核颗粒数在正常子宫内膜(2．5±2．0)、子宫内膜增殖症(2．9±2．0)、子宫内膜非典型增生(3．0±2．1)间相比,无统计学意义(P＞0．05)；在子宫内膜样腺癌(7．4±2．2)中,AgNOR颗粒数明显升高,与其余各组比,差异有统计学意义(P＜0．05)；AgNOR颗粒计数与子宫内膜样腺癌的分化程度(高分化4．7±2．3、中分化5．4±3．0、低分化7．5±2．1)、临床分期(Ⅰ期3．7±3．2、Ⅱ期4．5±2．9、Ⅲ期7．3±2．8、Ⅳ期7．8±2．3)、淋巴结转移(无转移3．6±3．3、有转移7．7±2．4)密切相关(P＜0．05)；AgNOR颗粒的形态分布随着子宫内膜病变的进展由单一型转变为弥散型。结论：AgNOR的每核颗粒数及其形态分布与子宫内膜样腺癌的发生、发展密切相关；检测AgNOR的颗粒数对早期诊断子宫内膜样腺癌、判断疾病预后有一定的临床价值。Objective： To investigate the usage of AgNOR in early diagnosis and prognosis ofendometrial adenoearcinoma. Methods： A total of 107 cases were collected in experimental group, including 25 ofendometrial hyperplasia, 19 of atypical proliferation and 63 of endometrial adenocarcinoma, and 25 cases of normal endometrium as control. Quantitative study and morphometric analysis of AgNOR were performed in different endometrial diseases with silver-staining technique. Results： AgNOR count in normal endometrium （2.5±2.0）, endometrial hyperplasia （2.9±2.0） and atypical proliferation （3.0±2.1） had no statistical differences（P 〉0.05）. In endometrial adenoeareinoma, AgNOR count increased obviously （7.4 ±2.2）, which was higher than that in endometrial hyperplasia group （2.9±2.0）, atypical proliferation group （3.0±2.1） and control group（2.5±2.0） prominently （P〈0.05）. AgNOR count increased with grade of cellular differentiation （4.7± 2.3, 5.4± 3.0, 7.5±2.1）, clinical phase （3.7±3.2, 4.5±2.9, 7.3±2.8, 7.8 ±2.3） and nodal metastasis （7.7±2.4, 3.6±3.3） （P〈0.05）. AgNOR image changed from simple type to dispersed type with the progression of disease. Conclusion： The quantitative evaluation and morphometrie analysis of AgNOR are related to the incidence of endometrial adenocarcinoma, and it can provide evidence for diagnosis ofendometrial adenocarcinoma and can be used as a prognostic index in malignancies.

关 键 词：AGNOR 子宫内膜病变 

分 类 号：R737.33[医药卫生—肿瘤]