Galectin-1基因在人孕早期绒毛和蜕膜组织中表达的研究  

Preliminary Study on the mRNA and Protein Expression of Galectin-1 Gene in Normal Villi and Decidua Tissue of the First Trimester

作　　者：孔紫靖[1] 胡丽芳[1] 李允光[1] 刘雪梅[1] 钟刚[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院妇产科,武汉430030

出　　处：《生殖与避孕》2007年第1期38-41,共4页Reproduction and Contraception

摘　　要：目的：探讨早孕绒毛和蜕膜组织中Galectin-1mRNA及蛋白的表达与胚胎发育和胎盘形成的关系。方法：对70例非意愿妊娠的6-12周正常早孕行人工流产者,按孕周分为7组,每组10例。收集其绒毛和蜕膜组织,分别采用半定量RT-PCR及Western blot方法检测Galectin-1mRNA和蛋白的表达水平。结果：在整个孕早期绒毛组织中都有Galectin-1mRNA的表达,孕6周表达最低,随孕周增加而逐渐增高,孕12周最高,Galectin-1蛋白表达与mRNA表达趋势一致；其在蜕膜组织中的表达与绒毛组织中类似。结论：Galectin-1mRNA和蛋白在早孕绒毛和蜕膜组织中的表达均随孕周增加而增高,提示该基因在早孕期胎盘形成过程中起重要作用。Objective： To study the mRNA and protein expression of galectin-1 gene in normal villi and decidua tissue during the first trimester and its relation to fetation and placentation. Methods： Semi - quantitative RT - PCR analysis and Western blot were used to detect the galectin-1 mRNA and protein expression levels in 70 cases of normal villi and decidua tissue, which were divided into 7 groups（10 in each group） according to the gestational weeks（6-12 weeks）. Results： The galectin-1 mRNA and protein were all expressed in villi and decidua tissue during the first trimester. Among them ,the expression of galectin-1 mRNA in 6 weeks of gestation was the lowest and the highest was in 12 weeks of gestation.Western blot result suggested that the protein expression increased with the progress of gestational weeks. Conclusion： The physiological changes of galectin-1 mRNA and protein expression in villi and decidua tissue suggest that galectin-1 gene may play an important role in placentation during the first trimester.

关 键 词：孕早期 绒毛 GALECTIN-1 基因表达 

分 类 号：R714.21[医药卫生—妇产科学]