颞叶癫大鼠海马区NCX3 mRNA和蛋白的表达变化  

Altered Expression of Na^+-Ca^(2+) Exchanger Isoform NCX3 mRNA and Protein in Hippocampus of Temporal Lobe Epileptic Rat

作　　者：江利敏[1] 肖波[1] 林祐成[1] 李蜀渝[1] 周艳辉[1] 柳茵[1] 

机构地区：[1]中南大学湘雅医院神经内科,410008

出　　处：《中国临床神经科学》2007年第1期1-7,共7页Chinese Journal of Clinical Neurosciences

基　　金：教育部高等学校优秀青年教师教学科研奖励计划(2001-182);高等学校博士学科点专项基金(20030533042)

摘　　要：目的:动态观察钠-钙交换体(NCX)mRNA和蛋白在氯化锂-匹罗卡品致模型大鼠海马CA1、CA3及齿状回区表达的变化,探讨其在癫发生发展中的作用。方法:用氯化锂-匹罗卡品制备癫动物模型;应用原位杂交和免疫组化技术检测各时间点NCX3mRNA和蛋白的表达。结果:急性期(6～24h)海马各区NCX3mRNA表达均随时间的延长逐渐减少;进入静止期各区表达趋向回升,慢性反复自发发作期(30、60d)各区表达又出现不同程度的两次下调。除致后6h大鼠海马各区的NCX3蛋白表达无明显变化外,NCX3蛋白变化趋势与NCX3mRNA基本一致。结论:NCX3表达下调可能通过增加神经元钙超载,改变海马神经元的兴奋性,促使癫发生。Aim： To investigate the expressions of Na^＋-Ca^2＋ exchanger isoform NCX3 mRNA and protein in the dentate gyms, CA1, CA3 regions by hippocampus of lithium-pilocarpine induced epileptic rat. Methods： The models of epilepsy were established by injecting pilocarpine and lithium. The NCX3 mRNA and protein were quantitatively analyzed at different time by using in situ hybridization and immunohistochemistry. Results： Expression of NCX3 mRNA in the hippocampus had significant decreases at 6 h, 12 h and 24 h after seizures, then increased slowly to normal level. However, at 30 d and 60 d, a second dramatic decrease was found in NCX3 mRNA expression. The change tendency of NCX3 protein was similar to NCX3 mRNA, except the protein did not increase at 6 h. Conclusion： The decreased expression of NCX3 in hippocampus might increase the overloading of free intracellular calcium and change the excitability of hippocampal neurons to induce seizures.

关 键 词：颞叶癫癇 海马 钠-钙交换体 

分 类 号：R742.1[医药卫生—神经病学与精神病学]